Molecular cytogenetic studies on the process of genomic and chromosomal reorganization in amphibians and reptiles

两栖类和爬行类基因组和染色体重组过程的分子细胞遗传学研究

• 批准号: 22370081
• 负责人: MATSUDA Yoichi
• 金额: $ 11.81万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370081/
• 关键词: 両生類; 爬虫類; FISH; ゲノム重複; 核型進化; 染色体地図; マイクロ染色体; ゲノムの区画化; ネッタイ爪ガエル; 鳥類; 祖先核型; 羊膜類; マクロ染色体

To delineate the process of genomic and karyotypic evolution of tetrapods, we constructed comparative cytogenetic maps of Pelodiscus sinensis, Crocodylus siamensis, Elaphe quadrivirgata, Varanus salvator, Xenopus tropicalis, and Xenopus laevis. Our results provide the possibility that the ancestral karyotype of tetrapods had at least 10 large linkage groups of macrochromosomes and many microchromosomal pairs, which have been conserved in the chicken. We identified all nine homoeologous chromosome groups of X laevis, which were homologous within the same groups except for two inversions, and the genetic linkages were highly conserved between X. tropicalis and X. laevis except for one fusion and one inversion. In E. quadrivirgata, microchromosomal genes showed significantly higher GC content than macrochromosomal genes as in the chicken, suggesting that the chromosome size-dependent GC heterogeneity had already occurred before the lepidosaur-archosaur split, 275 million years ago.

为了描绘四足动物的基因组和核型进化过程，我们构建了中华鳖、暹罗鳄鱼、四头锦蛇、萨尔瓦多巨蜥、热带爪蟾和非洲爪蟾的比较细胞遗传学图谱。我们的结果提供了四足动物的祖先核型至少有10个大的大染色体连锁群和许多微染色体对的可能性，这些连锁群在鸡中是保守的。我们鉴定了非洲爪蟾的全部九个同源染色体组，除了两个倒位之外，它们在同一组内是同源的，并且除了一个融合和一个倒位之外，热带爪哇和非洲爪哇之间的遗传连锁高度保守。在 E.quadrivirgata 中，微染色体基因的 GC 含量显着高于鸡中的大染色体基因，这表明染色体大小依赖性的 GC 异质性在 2.75 亿年前的鳞龙-祖龙分裂之前就已经出现了。

项目成果

Comparative genomics : tracking chromosomal evolution in vertebrates

比较基因组学：追踪脊椎动物的染色体进化

• 发表时间: 2011
• 作者: 表迫竜也;西野敦雄;西田宏記;Tanaka Kenya;Matsuda Y
• 通讯作者: Matsuda Y

核型進化が語るもの-マイクロ染色体とゲノム構造の区画化-

核型进化告诉我们什么 - 微染色体和基因组结构的划分 -

• 发表时间: 2011
• 作者: Fujishima;Masahiro;松田洋一
• 通讯作者: 松田洋一

Additional copies of CBX2 in the genomes of males of mammals lacking SRY, the Amami spiny rat (Tokudaia osimensis) and the Tokunoshima spiny rat (Tokudaia tokunoshimensis)

• DOI: 10.1007/s10577-011-9223-6
• 发表时间: 2011-07-01
• 期刊: CHROMOSOME RESEARCH
• 影响因子: 2.6
• 作者: Kuroiwa, Asato;Handa, Sanae;Matsuda, Yoichi
• 通讯作者: Matsuda, Yoichi

The process of a Y-loss event in an XO/XO mammal, the Ryukyu spiny rat

• DOI: 10.1007/s00412-010-0275-8
• 发表时间: 2010-10-01
• 期刊: CHROMOSOMA
• 影响因子: 1.6
• 作者: Kuroiwa, Asato;Ishiguchi, Yasuko;Matsuda, Yoichi
• 通讯作者: Matsuda, Yoichi

Dynamics of karyotypic evolution in squamate reptiles inferred comparative gene mapping

有鳞爬行动物核型进化动态推断比较基因图谱

• 发表时间: 2013
• 作者: Srikulnath K;Matsubara K;Uno Y;Nishida C;Matsuda Y
• 通讯作者: Matsuda Y