Effects of intermediate frequency magnetic fields on central nervous system and immune systems in mice.

中频磁场对小鼠中枢神经系统和免疫系统的影响。

基本信息

批准号：
22300255
负责人：
KUNUGITA Naoki
金额：
$ 12.23万
依托单位：
National Institute of Public Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

项目摘要

Recently, a cooking appliance based on the principle of electromagnetic induction has come to be used domestically on a widespread basis; this induction heating cooking hob mainly generates intermediate-frequency magnetic fields (IF-MF). However, whether electromagnetic fields originating from household appliances represent a health risk remains uncertain. We investigated the effect of IF-MF on the expressions of memory function-related genes and related transduction molecules in the mouse hippocampus. Male and female C57BL/6J mice were allotted to a control (sham-exposed), an exposure, or a recovery (one week after exposure) group and were exposed to IF-MF (21 kHz, 3.8 mT) one hour per day for 2 weeks. Twenty-four hour after final exposure, the expression levels of memory function-related genes and the mRNA levels for signal transduction pathway molecules in the hippocampi were examined using real-time RT-PCR. The relative mRNA expression levels of the N-methyl-D aspartate (NMDA) receptor subunits NR1, NR2A, and NR2B as well as transcription factors (calcium/calmodulin-dependent protein kinase [CaMK] -IV, cyclic AMP responsive element binding protein [CREB] -1) and neurotrophins (nerve growth factor [NGF], and brain-derived neurotrophic factors [BDNF]) were not significantly altered in the IF-MF-exposed mice. We also examined the morphology of the hippocampus using a histological analysis, but no changes in the IF-MF-exposed mice were seen. The present findings suggest that IF-MF exposure may not affect cognitive function in the present animal model.

最近，基于电磁诱导原理的烹饪设备已在国内广泛使用。这种感应加热烹饪霍布主要产生中间频率磁场（IF-MF）。但是，源自家用电器的电磁场是否代表健康风险仍然不确定。我们研究了IF-MF对小鼠海马中记忆功能相关基因和相关转导分子的表达的影响。将男性和雌性C57BL/6J小鼠分配给对照（假暴露），暴露或恢复（暴露后一周）组，并暴露于IF-MF（21 kHz，3.8吨）每天一小时一小时，持续2周。最终暴露后二十四小时，使用实时RT-PCR检查了海马中的信号转导途径分子的记忆功能相关基因的表达水平和MRNA水平。 The relative mRNA expression levels of the N-methyl-D aspartate (NMDA) receptor subunits NR1, NR2A, and NR2B as well as transcription factors (calcium/calmodulin-dependent protein kinase [CaMK] -IV, cyclic AMP responsive element binding protein [CREB] -1) and neurotrophins (nerve growth factor [NGF], and brain-derived在IF-MF暴露的小鼠中，神经营养因子[BDNF]）没有显着改变。我们还使用组织学分析检查了海马的形态，但是没有看到IF-MF暴露的小鼠的变化。目前的发现表明，如果MF暴露可能不会影响本动物模型中的认知功能。