The creation of the supramolecular chromoprotein trimer unit sensible color RGB light

超分子色素蛋白三聚体单元感测彩色RGB光的创建

• 批准号: 21651047
• 负责人: TOMIOKA Hiroaki
• 金额: $ 2.14万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21651047/
• 关键词: ナノ材料創製; バクテリオロドプシン; 3量体; ナノ材料; 超分子; ナノバイオ; 蛋白質; RGB; 色覚色素

Human color vision is mediated by three cone receptors in retina. Cone cells contain photosensitive protein molecules which consist of the protein opsin linked to retinal (vitamine A aldehyde), a prosthetic group. The three distinct photoreceptor proteins have absorption maxima(λmax)at 560, 530 and 426 nm respectively. These absobances correspond to the red(R), green(G)and blue(B)regions of the spectrum. The photoexcited cone pigments are degraded to opsins and retinals. These are unstable and unable to apply to photosensitive divices.Extremely halophilic archaebacteria have photosensitive proteins homologous with rhodopsin. These bacterial rhodopsin-like proteins are not photodegraded and extremely stable. One of the bacterial rhodopsin, bacteriorhodopsin(bR)forms trimer in the cell membrane. The absorption maximum for bR is 560 nm identical to that for the red cone pigment. If the stable chromoproteins withλmax at 530 and 426 nm is able to prepared, it is possible to challenge to make the mixed trimer unit consisting of three distinct chromoproteins with absorbances corresponding to RGB regions. The creation of the artificial proteins with 80-nm blue shifted λmax is successful.

人类的色觉是由视网膜中三个锥体受体介导的。锥细胞含有光敏蛋白分子，这些分子由与视网膜（维生素A醛）相关的蛋白Opsin组成。三种不同的光感受器蛋白分别在560、530和426 nm处具有后悔最大值（λmax）。这些抗击对应于频谱的红色（r），绿色（g）和蓝色（b）区域。光激发锥体色素被降解为Opsins和退休。这些是不稳定的，无法应用于光敏的划分。高淡淡淡卤素蛋白具有光敏感蛋白与Rhodopsin同源。这些细菌视紫红质样蛋白不是光降解且极为稳定的。细菌视紫红质之一，细菌近红淡曲（BR）在细胞膜中形成三聚体。 BR的最大吸收最大为560 nm，与红色锥色素相同。如果在530和426 nm处具有λmax的稳定铬蛋白能够制备，则有可能挑战混合触发单元，由三种不同的具有与RGB区域相对应的吸光度的不同染色体组成。用80 nm蓝色移动的λmax创建人造蛋白是成功的。