Establishment of tracking method for induced pluripotent stem cells using 64-Cu positron emitting isotope

64-Cu正电子发射同位素诱导多能干细胞追踪方法的建立

• 批准号: 21791171
• 负责人: NAKAJIMA Takahito
• 金额: $ 2.58万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791171/
• 关键词: iPS細胞; 64-Cu; ポジトロン核種; 放射線; 再生医学; 細胞・組織

Induced pluripotent stem cells (iPSCs) Labeling by 64-Cu positron emitting isotopes is a technical success with using PTSM material as a binding material. And positron emission tomography (PET) images which indicate iPSC distributions were acquired successfully. Two administration pathways were used ; 1) intravenous injection via tail vein, 2) left ventricle injection directly. There was significant difference between each distribution. Only lung distribution was seen by intravenous injection. On the other hand, the distributions to brain, kidneys, and transplanted tumors were described. Since half-life for 64-Cu is 13 hours, PET images could be acquired at 24 hours after labeled cells injection. Comparison with images for 18F-FDG labeled iPSCs, 64-Cu labeled image has less signal-to-noise ratio than that of 18F-FDG within 6 hour after positron-labeled cells administration.

诱导多能干细胞 (iPSC) 通过 64-Cu 正电子发射同位素进行标记是使用 PTSM 材料作为结合材料的技术成功。并成功获取了表明 iPSC 分布的正电子发射断层扫描 (PET) 图像。使用两种给药途径； 1)尾静脉静脉注射，2)直接左心室注射。各分布之间存在显着差异。静脉注射仅观察到肺部分布。另一方面，描述了脑、肾和移植肿瘤的分布。由于 64-Cu 的半衰期为 13 小时，因此可以在注射标记细胞后 24 小时获取 PET 图像。与 18F-FDG 标记的 iPSC 图像相比，在正电子标记细胞给药后 6 小时内，64-Cu 标记图像的信噪比低于 18F-FDG 的图像。

项目成果

In vivo tracking of 18F-FDG-labeled induced pluripotent stem cells to tumor-bearing mice

18F-FDG 标记的诱导多能干细胞对荷瘤小鼠的体内追踪

• 发表时间: 2009
• 作者: Takahito Nakajima;Hirohumi Hanaoka;Keigo Endo
• 通讯作者: Keigo Endo

lnvivo tracking of 18F-FDG-labeled induced pluripotent stem cells to tumor-bearing mice

18F-FDG 标记的诱导多能干细胞对荷瘤小鼠的体内追踪

• 发表时间: 2009
• 作者: 中島崇仁;花岡宏史;遠藤啓吾
• 通讯作者: 遠藤啓吾

骨生検針のトラブル時における対処方法の紹介

介绍如何处理骨活检针的问题

• 发表时间: 2010
• 作者: 岡本嘉一;國松聡;河野達夫;那須克宏;新津守;南学;中島崇仁
• 通讯作者: 中島崇仁