Decomposition of dyes by Bilirubin Oxidase

胆红素氧化酶分解染料

基本信息

批准号：
13680130
负责人：
KATAYAMA Michiko
金额：
$ 2.3万
依托单位：
Tokyo Kasei University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

In order to examine the decolonzation of dyeing effluent by enzyme, aqueous solutions of ten commercial anthraquinone dyes and eight commercial azo dyes were treated with Bilirubin oxidase (BO). Efficient decolorization ofall anthraquinone dyes was confirmed by measuring the visible and UV absorption spectra of the dye solution to which the enzyme was added.An aqueous solution of purified C.I. Acid Blue 40, to ^hich the enzyme wasadded, was shaken at 40 ℃.Aliquots of the solution after the specified time were analyzed by HPLC after removing the enzyme. Thechromatograms showed that the decomposition of dye (C.I. Acid Blue 40) started immediately after the addition of the enzyme. A peak around a retention time of 5.2 min,which can be related to the decomposition complex, increased gradually after the addition of the enzyme and was reached after about 3 h. That peak was nearly maintained even after 24 h, although it gradually decreased. Theabsorption spectrum of the product at that peak was determined by a photodiode array analysis.Efficient decolorization of six azo dyes was confirmed by measureing the visible and tJV absorption spectraof the dye solution in the presence of the enzyme, but that of the two azo dyes, C.I. Direct Black 22 and C.I.Direct Violet 48 was not efficient. Also HPLC was introduced to measure decolorization of dyes. The chromatograms showed that the decomposition of the eight dyes started immediately after the addition of the enzyme. The decolorization ratios calculated from the peak areas in the chromatograms were ca. 100% for first the six dyes and 90% for that last two dyes.

为了检查酶对染色废水的脱色作用，用胆红素氧化酶 (BO) 处理 10 种市售蒽醌染料和 8 种市售偶氮染料的水溶液，通过测量染料的可见光和紫外吸收光谱证实了所有蒽醌染料的有效脱色。添加了酶的染料溶液。纯化的 C.I.酸性蓝 40 的水溶液，其中含有酶加入酶，在40℃下摇动。在除去酶后，在规定的时间后对溶液的等分试样进行分析。色谱图显示，在加入酶后，染料(C.I.酸性蓝40)立即开始分解。保留时间为5.2分钟，这可能与分解复合物有关，添加酶后逐渐增加，并在约3小时后达到该峰值，甚至在加入酶后也几乎保持该峰值。 24小时，尽管通过光电二极管阵列分析测定了该峰处产物的吸收光谱。通过在酶存在下测量染料溶液的可见光和tJV吸收光谱证实了六种偶氮染料的有效脱色。，但两种偶氮染料 C.I. Direct Black 22 和 C.I.Direct Violet 48 的脱色效果并不理想。色谱图显示，添加酶后，八种染料立即开始分解。根据色谱图中的峰面积计算，前六种染料的脱色率约为 100%，后两种染料的脱色率约为 90%。