Elucidation of follicle selection system using bovine follicle culture and application for follicle and oocyte culture of wildlife

利用牛卵泡培养阐明卵泡选择系统及其在野生动物卵泡和卵母细胞培养中的应用

• 批准号: 21780286
• 负责人: NAGANO Masashi
• 金额: $ 2.66万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780286/
• 关键词: 臨床繁殖; 産科; 体外受精; 卵胞発育; 猫科動物; 獣医学; 動物; 卵胞; 卵子; 体外培養; 臨床繁殖; 産科; 体外受精; 卵胞発育; 猫科動物; 獣医学; 動物; 卵胞; 卵子; 体外培養

For clarification of mechanisms of follicular development and degeneration, growing oocytes were collected from bovine and feline ovaries and cultured in vitro. It was suggested that high concentration of follicular stimulating hormone inhibited the oocyte growth in vitro, and estradiol was essential for oocyte and follicular development. Fibroblast growth factor 7 stimulated oocyte growth and granulosa cell proliferation in early culture stage, but it did not have previous effects on growing oocytes in late culture stage.

为了阐明卵泡发育和变性的机制，从牛和猫卵巢中收集生长的卵母细胞，并在体外培养。有人提出，高浓度的卵泡刺激激素抑制了体外卵母细胞的生长，而雌二醇对于卵母细胞和卵泡发育至关重要。成纤维细胞生长因子7在早期培养阶段刺激了卵母细胞的生长和颗粒细胞的增殖，但它以前对晚期培养阶段的卵母细胞的影响没有影响。