Analysis of the relationship between herbicide resistance mechanism and biophoton generation

除草剂抗性机制与生物光子产生关系分析

• 批准号: 21780018
• 负责人: NUKUI Hideki
• 金额: $ 1.83万
• 依托单位: Shizuoka Prefectural Research Institute of Agriculture and Forestry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780018/
• 关键词: 除草剤抵抗性; バイオフォトン

The generating mechanism of herbicide responsive photon emission from cultured rice cells was analyzed. 50-100 ppm bensulfronmethyl (BSM) treatment induced an increment of photon emission from rice cells. Emission patterns of 18 tested herbicides were different from each other. CYP81A6, a cytochrome P450 gene reported to be responsible for BSM tolerance in rice, was isolated from the rice cells, and was silenced by RNAi. Responses of CYP81A6-silenced cell line to BSM and branched chain amino acids revealed that the BSM detoxifying reaction by cytochrome P450 is involved in BSM responsive photon emission from rice cells.

分析了水稻细胞除草剂响应光子发射的产生机制。 50-100 ppm 苯磺隆 (BSM) 处理可诱导水稻细胞的光子发射增加。 18 种测试的除草剂的排放模式彼此不同。 CYP81A6 是一种细胞色素 P450 基因，据报道与水稻中的 BSM 耐受性有关，它是从水稻细胞中分离出来的，并通过 RNAi 使其沉默。 CYP81A6 沉默细胞系对 BSM 和支链氨基酸的反应表明，细胞色素 P450 的 BSM 解毒反应参与了水稻细胞的 BSM 响应光子发射。

项目成果

Involvement of cytochrome P450 in bensulfronmethyl responsive photon emission from rice cells

细胞色素 P450 参与水稻细胞苯磺隆响应光子发射

• 发表时间: 2011
• 作者: Nukui;H.;Iyozumi;H.;Kato;K.;Kageyama;C.
• 通讯作者: C.

イネ培養細胞の除草剤応答発光

培养水稻细胞的除草剂响应发光

• 发表时间: 2010
• 作者: Nukui;H.;貫井秀樹
• 通讯作者: 貫井秀樹