Gene expression profile of small endometrial cancer by using gene expression array and laser capture microdissection

使用基因表达阵列和激光捕获显微切割研究小型子宫内膜癌的基因表达谱

• 批准号: 13671759
• 负责人: SUGIYAMA Yuko
• 金额: $ 1.41万
• 依托单位: Japanese Foundation for Cancer Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671759/
• 关键词: Endometrial Cancer; Laser capture microdissection; gene expression array; Gene expression profiling; 遣伝子発現解析

The gene expression array method enables us to achieve an expression pro filing with thousands of genes. Clinically resected bulk cancer tissues, however, contain not only cancer cells but also stromal cells, which may affect gene expression profiling and hamper accurate analysis of the cancer cells per se. Therefore, a procedure for dissecting specific cells, such as laser capture microdissection, is needed for the clinical application of a gene expression array. There has been no study actually comparing two gene expression profiles; that obtained using RNA extracted from cancer cells by laser capture microdissection and that obtained using RNA extracted from bulk cancer tissues. Here, we first demonstrated the difference in expression patterns between them, without any amplification procedures. In addition, differential expression analysis between tumor and non-tumor tissue yielded quite different patterns between the two methods. We concluded that microdissection is essential for gene expression pro filing of clinical specimens.Then we analyzed the difference in the pattern of gene expression between the tumor and normal endometrial cells in small endometrial cancer. Sixty-nine genes were up-regulated and ninty-nine genes were down-regulated in the tumor cells when compared with the normal endometrial cells.

基因表达阵列方法使我们能够获得数千个基因的表达谱。然而，临床上切除的大块癌组织不仅含有癌细胞，还含有基质细胞，这可能会影响基因表达谱并妨碍对癌细胞本身的准确分析。因此，基因表达阵列的临床应用需要一种用于解剖特定细胞的程序，例如激光捕获显微切割。目前还没有研究真正比较两种基因表达谱；一种是使用通过激光捕获显微切割从癌细胞中提取的 RNA 获得的，另一种是使用从大块癌组织中提取的 RNA 获得的。在这里，我们首先展示了它们之间表达模式的差异，没有任何扩增程序。此外，肿瘤和非肿瘤组织之间的差异表达分析在两种方法之间产生了截然不同的模式。我们得出结论，显微切割对于临床标本的基因表达谱分析至关重要。然后我们分析了小子宫内膜癌中肿瘤细胞和正常子宫内膜细胞之间基因表达模式的差异。与正常子宫内膜细胞相比，肿瘤细胞中有 69 个基因上调，99 个基因下调。

项目成果

Yuko Sugiyaina: "Microdissection is Essential for Gene Expression Profiling of Clinically Resected Cancer Tissues"American journal of clinical pathology. 117巻・1号. 109-116 (2002)

Yuko Sugiyaina：“显微解剖对于临床切除的癌症组织的基因表达谱分析至关重要”美国临床病理学杂志第 117 卷，第 1 期 109-116 (2002)。