Analyses of the regulation of the chromatin structure and transcriptional activity using in vitro reconstitution system

利用体外重构系统分析染色质结构和转录活性的调控

• 批准号: 21770167
• 负责人: HIZUME Kohji
• 金额: $ 2.91万
• 依托单位: National Institute of Genetics (2010-2011)Kyoto University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770167/
• 关键词: タンパク質; 核酸の構造・動態・機能; クロマチン; ヌクレオソーム; 原子間力顕微鏡; DNA複製; 原子力間顕微鏡; 試験管内再構成; タンパク質; 核酸の構造・動態・機能; クロマチン; ヌクレオソーム; 原子間力顕微鏡; DNA複製; 原子力間顕微鏡; 試験管内再構成

In order to reveal the structure induced by specific factors such as histone-tail modifications or chromosomal proteins, we observed the reconstituted chromatin under the various conditions.(1) hyper-acetylation of histone-tails causes stronger repulsive force between nucleosomes and the thinner H1-induced fiber than non-acetylated chromatin.(2) Sliding and dissociation of nucleosomes were observed by fast-AFM.(3) ORC(origin recognition complex) associated with nucleosome and formed larger complex.

为了揭示由特定因素（例如组蛋白尾巴修饰或染色体蛋白）诱导的结构，我们观察到在各种条件下重构染色质的重构染色质。（1）组蛋白尾巴的高乙基化导致核小体之间的压抑性在核小体和稀薄的H1诱导的纤维纤维之间比未非核酸固定的核酸元素和固定素的固定蛋白（2）。快速AFM。（3）与核小体相关的ORC（原点识别复合物）并形成更大的复合物。