The effects of repetitive transcranial magnetic stimulation on neurogenesis

重复经颅磁刺激对神经发生的影响

• 批准号: 13671001
• 负责人: UKAI Satoshi
• 金额: $ 0.7万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671001/
• 关键词: TMS; neurogenesis; HFOs; SEPs; 神経細胞新; うつ病

The basis for therapeutic effect and optimum stimulus condition of rTMS (repetitive transcranial magnetic stimulation) is still unknown. We investigated the therapeutic mechanisms of rTMS in the light of neurogeneais in the dentate gyrus of the rats and the modulation of HFOs (high frequency oscillations) in the healthy human subjects.1. We aimed to examine the possibility that rTMS triggers hippocampal neurogenesis, and if so, to obtain optimum rTMS stimulus condition using neurogenesis as an indicator. Rats were given either real or sham rTMS. For the real rTMS group, rTMS was administered (10 Hz, 150 pulses, 120% motor threshold /day) for 14 days and the two groups were compared. The effects of rTMS .on neurogenesis in the dentate gyrus using bromodeoxyuridine (BrdU) immunohistochemistry, which identifies newly generated cells. Cells were labeled for neuronal nuclear protein (NeuN) to identify neurons.2. HFOs of SEPs (somatosensory evoked potentials) in 7 healthy human subjects were recorded before and after the slow rTMS on the primary sensory area (0.5 Hz, 50 pulses, 80% motor threshold). 6 of the 7 subjects showed significant increase of the amplitude and the power of HFOs after rTMS, while no subjects showed after sham rTMS. These results suggest that slow rTMS might directly increase the activity of GABAergic inhibitory interneurons in the cortex and suppress cortical excitability through these inhibitory interneurons. Studies of HFOs changes with other rTMS conditions are needed to obtain the optimum stimulus condition.

rTMS（重复经颅磁刺激）的治疗效果和最佳刺激条件的基础仍不清楚。我们根据大鼠齿状回的神经发生和健康人类受试者的 HFO（高频振荡）调节研究了 rTMS 的治疗机制。 1．我们的目的是检查 rTMS 触发海马神经发生的可能性，如果是的话，以神经发生为指标获得最佳 rTMS 刺激条件。对大鼠进行真实或假的 rTMS 治疗。对于真实 rTMS 组，给予 rTMS（10 Hz、150 脉冲、120% 运动阈值/天）14 天，并对两组进行比较。使用溴脱氧尿苷 (BrdU) 免疫组织化学分析 rTMS 对齿状回神经发生的影响，该免疫组织化学可识别新生成的细胞。细胞标记神经元核蛋白（NeuN）以识别神经元。 2．记录了 7 名健康受试者在初级感觉区（0.5 Hz、50 个脉冲、80% 运动阈值）进行慢速 rTMS 前后的 SEP（体感诱发电位）的 HFO。 7 名受试者中有 6 名在 rTMS 后表现出 HFO 的振幅和功率显着增加，而在假 rTMS 后没有受试者表现出明显增加。这些结果表明，慢速 rTMS 可能会直接增加皮质中 GABA 能抑制性中间神经元的活性，并通过这些抑制性中间神经元抑制皮质兴奋性。需要研究 HFO 随其他 rTMS 条件的变化以获得最佳刺激条件。

项目成果

Hiroshi Mori: "Expression of mouse igf2mRNA-binding Protein 3 and its implication for the developing central nervous system"Journal of Neuroscience Research. 64. 132-143 (2001)

Hiroshi Mori：“小鼠 igf2mRNA 结合蛋白 3 的表达及其对中枢神经系统发育的影响”神经科学研究杂志。

Shunsuke Kawaguchi: "Interictal spikes in the fusiform and inferior temporal gyri of an epileptic patient with colored elementary visual auras : a 5-year longitudinal MEG ECD study"Neuroreport. 14. 637-640 (2003)

Shunsuke Kawaguchi：“具有彩色基本视觉先兆的癫痫患者的梭状回和颞下回的发作间期尖峰：一项为期 5 年的纵向 MEG ECD 研究”Neuroreport。

K.Hirata, Y.Koga, K.Nagata, K.Yamazaki: "Recent Advances in Human Brain Mapping"Elsevier. 936 (2002)

K.Hirata、Y.Koga、K.Nagata、K.Yamazaki：“人类大脑图谱的最新进展”爱思唯尔。

Satoshi Ukai, Kazuhiro hinosaki, Ryouhei Ishii, Yuko-Mizuno atsumoto, Tsuyoahi Inouye, Norio Hirabuki, Toshiki Yoshimine, SE Robinson, Masatoshi Takeda In K. Miyoshi, CM Shapiro, M Gaviria, Y Morita (Eds.): "MEG functional neuroimaging of schizophrenic pa

Satoshi Ukai、Kazuhiro hinosaki、Ryouhei Ishii、Yuko-Mizuno atsumoto、Tsuyoahi Inouye、Norio Hirabuki、Toshiki Yoshimine、SE Robinson、Masatoshi Takeda In K. Miyoshi、CM Shapiro、M Gaviria、Y Morita（编辑）：“MEG 功能神经影像学

Satoshi Ukai, Kazuhiro hinosaki, Ryouhei Ishii, Asao Ogawa, Yuko Mizuno-Matsumoto, Tuyoshi Inouye, Norio Hirabuki, Toshiki Yoshimine, S.E. Robinson, Masatoshi Takeda, In K. Hirata, Y. Koga, K. Nagata, K. Yamazaki (Eds.): "Spatially Filtered MEG Analysis E

Satoshi Ukai、Kazuhiro hinosaki、Ryouhei Ishii、Asao Okawa、Yuko Mizuno-Matsumoto、Tuyoshi Inouye、Norio Hirabuki、Toshiki Yoshimine、S.E.