Development of early diagnosis for obesity and diabetes using insulin signaling gene expressions in dogs and cats

利用狗和猫的胰岛素信号基因表达开发肥胖和糖尿病的早期诊断

• 批准号: 21380195
• 负责人: ARAI Toshiro
• 金额: $ 11.4万
• 依托单位: Nippon Veterinary and Life Science University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380195/
• 关键词: 動物; 肥満; 糖尿病; 遺伝子; インスリン; 早期診断

Early diagnostic method for obesity and diabetes mellitus using insulin signaling gene was investigated in dogs and cats. Expression of insulin signaling genes, insulin receptor substrate(IRS)-1,-2 and phosphatidylinositol kinase(PI3K), decreased significantly at the early stage of obesity in dogs and cats. Changes in plasma cholesterol profiles and adiponectin concentrations and peripheral leukocytes adiponectin receptor(ADIPOR) gene expression were also significant in obese and diabetic animals. IRS-2, PI3K and ADIPOR mRNA expression and plasma cholesterol profiles and adiponectin concentrations appeared to be potential diagnostic markers for obesity and diabetes in dogs and cats.

使用胰岛素信号转导基因的肥胖和糖尿病的早期诊断方法在狗和猫中研究了。胰岛素信号基因，胰岛素受体底物（IRS）-1，-2和磷脂酰肌醇激酶（PI3K）的表达在狗和猫的早期阶段显着降低。血浆胆固醇谱和脂联素浓度以及周围白细胞脂联素受体（adipor）基因表达的变化在肥胖和糖尿病动物中也很重要。 IRS-2，PI3K和ADIPOR mRNA表达以及血浆胆固醇谱和脂联素浓度似乎是狗和猫的肥胖和糖尿病的潜在诊断标记。

项目成果

Supplementing transglucosidase with a high-fiber diet for prevention of postprandial hyperglycemia in streptozotocin-induced diabetic dogs

• DOI: 10.1007/s11259-010-9342-0
• 发表时间: 2010-02-01
• 期刊: VETERINARY RESEARCH COMMUNICATIONS
• 影响因子: 2.2
• 作者: Sako, Toshinori;Mori, Akihiro;Arai, Toshiro
• 通讯作者: Arai, Toshiro

Rapid and reliable genotyping techniques for GM1 gangliosidosis in Shiba dogs by real time polymerase chain reaction with TaqMan minor groove binding probes

通过使用 TaqMan 小沟结合探针的实时聚合酶链反应对柴犬 GM1 神经节苷脂沉积症进行快速可靠的基因分型技术

• 发表时间: 2010
• 期刊: Journal of Diagnostic Investigation 22
• 作者: Chang H-S;Arai T;Yabuki A;Yamato O;et al
• 通讯作者: et al

Serum glycagted albumin as a glycemic control marker in diabetic cats

血清糖化白蛋白作为糖尿病猫的血糖控制标志物

• 发表时间: 2009
• 期刊: Journal of Veterinary Diagnostic Investigation 21
• 作者: Mori A;Lee P;Arai T;et al
• 通讯作者: et al

Clinical significance of insulin and adiponectin in obese animals

胰岛素和脂联素在肥胖动物中的临床意义

• 发表时间: 2010
• 作者: 三好猛晴,辻尚利,Khyrul Islam,八田岳 士,Abdul Alim,山地佳代子,Anisuzzaman;藤崎幸藏;Toshiro Arai
• 通讯作者: Toshiro Arai

Insulin treatment-induced daily changes to plasma adiponectin and TNF-α level and lipid metabolism parameters in dogs suffering from type 1 diabetes mellitus

胰岛素治疗引起的 1 型糖尿病犬血浆脂联素和 TNF-α 水平以及脂质代谢参数的日常变化

• DOI: 10.3923/ajava.2011.844.850
• 发表时间: 2011
• 期刊: Asian Journal of Animal and Veterinary Advances
• 作者: Mori N;Lee P;Yamamoto I;Nozawa S;Arai T
• 通讯作者: Arai T