Immobilization of cell adhesive protein on dental implant

细胞粘附蛋白在牙种植体上的固定化

• 批准号: 12671904
• 负责人: HAYAKAWA Tohru
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671904/
• 关键词: dental implant; titanium implant; cell-adhesive protein; epithelial attachment; tresyl chloride; fibronectin; XPS; surface treatment; 歯科用インプラント; 細胞接着因子; 固定化; チタン; ハイドロキシアパタイト

The aim of this study is to bound fibronectin directly on tresyl chloride (2, 2, 2 -trifluoroethanesulfonyl chloride) treated titanium surface for the development of tight connection of dental implant to gingival and/or epithelial tissue. Basic terminal OH groups of mirror polished titanium were reacted with tresyl chloride at 37 ℃ for 2 days. Tresylated titanium disk was immersed into the fibronectin/PBS solution for 24 h at 37 ℃.The activation reaction of basic OH of titanium with tresyl chloride was confirmed by F1s and O1s spectra in the measurement of X-ray photoelectron spectroscopy (XPS) and -O-S-O_2 bonds in the measurement of the Fourier transform infrared Reflection-Absorption spectrometer (FT-IR-Ras). XPS spectra after the reaction of fibronectin with titanium demonstrated the remarkable effect of the activation of terminal OH groups with tresyl chloride treatment. N1s peak derived from attached fibronectin was still remaining after 60 s argon-ion etching on the tresylated titanium. On the contrary N1s peak of the specimen without tresyl chloride treatment was almost disappeared after only 10 s argon-ion etching. Direct attachment of fibronectin to tresylated titanium was not removed after 60 s ultrasonic cleaning.Fibronectin, well-known cell-adhesive proteins, could be easily attached on thetitanium surface using tresyl chloride activation technique.

本研究的目的是将纤连蛋白直接结合在经三氟乙烷磺酰氯（2,2,2-三氟乙磺酰氯）处理的钛表面上，以形成牙种植体与镜面抛光的牙龈和/或上皮组织的紧密连接。将钛与三氟乙磺酰氯在37℃下反应2天。纤连蛋白/PBS溶液在37 ℃下反应24 h。X射线光电子能谱(XPS)测量中的F1s和O1s光谱以及钛中的-O-S-O_2键的测定证实了钛的碱性OH与三乙磺酰氯的活化反应。反应后傅里叶变换红外反射吸收光谱仪（FT-IR-Ras）的测量。含钛的纤连蛋白显示，经过三氟磺酰氯处理的末端OH基团的活化作用在对三磺酰基钛进行60秒的氩离子蚀刻后，源自附着的纤连蛋白的N1s峰仍然保留，相反，不含三磺酰氯的样品的N1s峰仍然存在。仅 10 秒的氩离子蚀刻后，氯化物处理就几乎消失了，纤连蛋白直接附着在其上。超声波清洗60秒后，三磺基化钛并未被去除。纤连蛋白，众所周知的细胞粘附蛋白，可以使用三氟磺酰氯活化技术轻松地附着在钛表面。

项目成果

谷本安浩, 安田清次郎, 早川 徹, 根本君也: "ハイドロキシアパタイトコーティングインプラントの有限要素方による応力解析-アパタイト層厚さおよび弾性率の影響-"日本口腔インプラント学会誌. 14・3. 414-422 (2001)

Yasuhiro Tanimoto、Seijiro Yasuda、Toru Hayakawa、Kimiya Nemoto：“通过有限元法对羟基磷灰石涂层种植体进行应力分析 - 磷灰石层厚度和弹性模量的影响 -”日本口腔种植学会杂志 14・3。 (2001)

Tohru Hayakawa, Kazuyo Kikutake, Kimiya Nemoto: "Conformational and Quantum Analysis of Dental Adhesive Carboxylic Acid and Carboxylic Acid Anhydride Monomers"Dental Materials Journal. 20・1. 1-15 (2001)

早川彻、菊竹和代、根宫：“牙科粘合剂羧酸和羧酸酐单体的构象和量子分析”牙科材料杂志 20・1。

Tohru Hayakawa, Masao Yoshinari, Hideo Kiba et al.: "Trabecular bond response to surface roughened and calcium phosphate (Ca-P) coated titanium implants"Biomaterials. 23・4. 1025-1031 (2002)

Tohru Hayakawa、Masao Yoshinari、Hideo Kiba 等：“对表面粗糙和磷酸钙 (Ca-P) 涂层的钛植入物的小梁粘合反应”23・4 (2002)。

Tohra Hayakawa,Kazuyo KiKutake,Kimiya Nemoto: "Conformational and Quantum Analysis of Dental Adhesive Carboxylic Acid and Carboxylic Acid Anhydride Monomers"Dental Materials Journal. 20(1)(印刷中). (2001)

Tohra Hayakawa、Kazuyo KiKutake、Kimiya Nemoto：“牙科粘合剂羧酸和羧酸酐单体的构象和量子分析”《牙科材料杂志》20(1)（印刷中）。

Tohru Hayakawa, Masao Yoshinari, Hideo Kiba et al.: "Trabecular bond response to surface roughened and calcium phosphate(Ca-P) coated titanium implants"Biomaterials. 23・4. 1025-1031 (2002)

Tohru Hayakawa、Masao Yoshinari、Hideo Kiba 等：“小梁结合对表面粗糙和磷酸钙 (Ca-P) 涂层的钛植入物的反应”23・4 (2002)。