AN ANALYSIS OF SYNAPTIC TRANSMISSION MECHANISM FROM TASTE RECEPTOR CELL TO GUSTATORY NERVE

味觉感受器细胞至味觉神经突触传递机制分析

• 批准号: 12671810
• 负责人: MIYAMOTO Takenori
• 金额: $ 1.98万
• 依托单位: NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671810/
• 关键词: Taste cell; Taste stmuli; transmitter; carbonfiber electrode; patch clamp; bioassay; paracrine; vallate papilla; von Ebner's gland complex; アミノ酸; アセチルコリン; ペプチド; 有廓乳頭味細胞; エブネル腺; 電気化学的検出法; セロトニン; グルタミン酸; マウス; 味神経; 塩味; うま味

Immunohistochemical studies have demonstrated that taste receptor cells containing serotonin exist in taste buds. In the previous studies, we detected serotonin secreted from the serosal side of the lingual epithelium in response to the repeated application of NaCl or citric acid to the mucosal side of the lingual epithelium using full-automatical perfusion/sampling device for epithelial tissue and HPLC. Therefore, we attempted to detect serotonin secreted from a single taste bud in response to the application of taste stimuli to the taste pore using localized stimulation procedure and carbon fiber electrodes. However, we were unable to detect any monoamines. We attempted to immunohistochemically detect localization of several subtypes of glutamatergic receptor molecules in the taste buds of the fungiform papilla. One of the metabotropic glutamatergic receptors, mGluR4 was detected in the taste bud cells, but not in the gustatory nerve terminal of the afferent synapse. Taken together w … More ith electrophysiological data, mGluR4 in the taste bud cells possibly play an inhibitory role for transduction mechanism of umami. These results suggest mat substances other than glutamate and monoamines mediate the transmission from taste receptor cells to gustatory nerves. Therefore, we performed a bioassay using vallate papilla (VP)/von Ebner's gland (VEG) complex, where several receptor molecules are expressed on the acinar cells. The results indicated that localized bitter stimuli to the cleft of isolated vallate papilla suppressed voltage-gated K^+ currents of acinar cells, whereas sour stimuli induced inward current responses in acinar cells with slow time course, suggesting that these actions of taste stimuli are due to paracrine effect of transmitters released from taste bud cells. These findings also suggest that transmitter released by bitter stimulus is different from that by sour stimulus, and receptor molecules for these multiple transmitters were expressed on acinar cells of VEG. Less

免疫组织化学研究表明，味蕾中存在含有血清素的味觉受体细胞。在之前的研究中，我们检测到舌上皮浆膜侧分泌的血清素，以响应对舌粘膜侧重复施加氯化钠或柠檬酸。因此，我们尝试使用全自动上皮组织灌注/采样装置和HPLC进行检测。使用局部刺激程序和碳纤维电极，单个味蕾响应味觉刺激而分泌血清素。然而，我们无法检测到任何单胺。我们尝试用免疫组织化学方法检测谷氨酸受体的几种亚型的定位。菌状乳头味蕾中的一种代谢型谷氨酸受体 mGluR4 在味蕾细胞中检测到，但在味蕾细胞中未检测到。结合电生理数据，味蕾细胞中的 mGluR4 可能对鲜味转导机制发挥抑制作用。这些结果表明，除了谷氨酸和单胺之外，还有其他物质介导味觉传递。因此，我们使用vallate乳头（VP）/von Ebner腺（VEG）复合物进行了生物测定，其中结果表明，对孤立的腭乳头裂隙的局部苦味刺激抑制了腺泡细胞的电压门控K^+电流，而酸味刺激则在缓慢的时间过程中诱导了腺泡细胞的内向电流反应。 ，表明味觉刺激的这些作用是由于味蕾细胞释放的递质的旁分泌作用所致。这些发现还表明，苦味刺激释放的递质与酸味刺激释放的递质不同，以及这些多种受体分子。递质在 VEG 的腺泡细胞上表达较少。

项目成果

Miyamoto et al.: "Differential transduction mechanisms underlying NaCl- and KCl-induced responses in mouse taste cells"Chemical Senses. vol.26. 67-77 (2001)

Miyamoto 等人：“小鼠味觉细胞中 NaCl 和 KCl 诱导反应的差异转导机制”Chemical Senses。

Miyazaki et al.: "Comparative study of the proliferative activity of serous-and mucous-type acinar cells in developing mongolian gerbil mixed salivary glands"Acta Histochemica et Cytochemica. Vol.35. 305-312 (2002)

Miyazaki等人：“蒙古沙鼠混合唾液腺中浆液型和粘液型腺泡细胞增殖活性的比较研究”组织化学与细胞化学学报。

Imendra 他: "Effect of acetic acid on intracellular Ca^ concentration in bullfrog taste disc cells"日本味と匂学会誌. 9巻3号. 531-534 (2002)

Imendra等人：“乙酸对牛蛙味盘细胞内Ca 2+ 浓度的影响”日本味觉学会杂志，第9卷，第3. 531-534期(2002)。

Miyamoto et al.: "MSG-induced response and its enhancement by GMP in mouse non-dissociated taste cells"Japanese Journal of Taste and Smell Research. vol.8. 307-310 (2001)

Miyamoto 等人：“小鼠非分离味觉细胞中味精诱导的反应及其通过 GMP 的增强”日本味觉和嗅觉研究杂志。

宮本武典他: "スナネズミのエブネル腺細胞の膜特性と味物質による修飾"歯科基礎医学雑誌. 44巻5号. 482 (2002)

Takenori Miyamoto 等人：“沙鼠埃布纳腺细胞的膜特性和味觉物质的修饰”《基础牙科医学杂志》第 44 卷，第 5. 482 期（2002 年）。