Genetic and physiological studies on seed coat cracking in soybean

大豆种皮开裂的遗传和生理研究

• 批准号: 21580009
• 负责人: TAKAHASHI Ryoji
• 金额: $ 3.16万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580009/
• 关键词: 大豆; 裂皮; 種子品質; 量的形質遺伝子座; DNAマーカー; QTL解析

The seed coat of soybean cracks under field conditions. Two QTLs, cr1(molecular linkage group(MLG) D1b and cr2 MLG M) were identified. The cr1 was not associated with seed size or maturity in near-isogenic lines(NILs). Therefore, markers around the QTL may be useful for breeding to reduce cracking symptoms without altering seed size or maturity. In contrast, two QTLs for net-like cracking(ncr1 and ncr2) were identified in MLG C1 with a distance of about 62 cM. These results suggest that genetic control of seed coat cracking occurring under field conditions was different from net-like cracking. Microarray of the NILs revealed genes highly expressed in the tolerant NIL(Gma. 26856, Gma. 16827, Gma. 173) and genes highly expressed in the sensitive NIL(Gma. 48109 and Gma. 49562).

大豆种皮在田间条件下会破裂。鉴定出两个QTL：cr1（分子连锁群（MLG）D1b和cr2 MLG M）。 cr1 与近等基因系 (NIL) 中的种子大小或成熟度无关。因此，QTL 周围的标记可能有助于育种，以减少开裂症状而不改变种子大小或成熟度。相比之下，MLG C1 中鉴定出两个网状开裂 QTL（ncr1 和 ncr2），距离约为 62 cM。这些结果表明，田间条件下发生的种皮开裂的遗传控制不同于网状开裂。 NIL 的微阵列揭示了在耐受性 NIL 中高表达的基因（Gma.26856、Gma.16827、Gma.173）和在敏感 NIL 中高表达的基因（Gma.48109 和 Gma.49562）。

项目成果

QTL analysis of net-like cracking in soybean seed coats

• DOI: 10.1270/jsbbs.60.28
• 发表时间: 2010-03
• 期刊: Breeding Science
• 影响因子: 2.4
• 作者: M. Oyoo;S. Githiri;E. Benitez;R. Takahashi

QTL Analysis of Seed Coat Cracking in Soybean

• DOI: 10.2135/cropsci2009.08.0440
• 发表时间: 2010-07-01
• 期刊: CROP SCIENCE
• 影响因子: 2.3
• 作者: Oyoo, Maurice E.;Benitez, Eduardo K.;Takahashi, Ryoji
• 通讯作者: Takahashi, Ryoji