Study of rejuvenation of aged oocyte "Aim at the establishment of the strategy of a new fertility treatment"

老化卵母细胞再生研究“旨在制定新的生育治疗策略”

• 批准号: 20591905
• 负责人: IGARASHI Hideki
• 金额: $ 2.91万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591905/
• 关键词: 生殖医学; マウス卵; 加齢; 小胞体ストレス; thapsigargin; tunicamycin; 若返り; BiP

Mitochondria (Mt) extracted from the hepatocyte to the purpose of the rejuvenation of the aged mouse were transplanted to ooplasm. However, neither the fertilization rate nor the embryonic development of the Mt transplant aged oocytes have been improved. The endoplasmic reticulum (ER) stress was examined as a new mechanism of aging of oocytes. An increase of the ER stress marker was confirmed in the aged mouse oocytes. The ER stress inducement material, thapsigargin, and tunicamycin decreased the fertilization rate after the in vitro fertilization of fresh mouse oocytes, and worsened the embryonic development. As for the above-mentioned result, the ER stress takes part in the aging of the oocyte, and overcoming the ER stress suggests that it be important in the rejuvenation of the aged oocyte.

从肝细胞提取的线粒体（MT）以衰老小鼠的恢复目的被移植至卵子。但是，MT移植老年卵母细胞的受精率和胚胎发育均未得到改善。内质网应力（ER）应力是卵母细胞衰老的新机制。在老年小鼠卵母细胞中证实了ER应力标记的增加。新鲜小鼠卵母细胞体外受精后，ER应力诱导材料，Thapsigargin和Gunsigamycin降低了受精率，并使胚胎发育恶化。至于上述结果，ER应力参与卵母细胞的衰老，并克服ER应力表明它对年龄卵母细胞的恢复活力很重要。

项目成果

マウス加齢卵におけるミトコンドリア機能の評価とミトコンドリア移植の試み

老年小鼠卵子线粒体功能评估及线粒体移植尝试

• 发表时间: 2008
• 作者: 五十嵐秀樹;高橋俊文;網田光善;倉智博久
• 通讯作者: 倉智博久

Roles of prostagrandins during oocytes maturation : lessons from knockout mice.

前列腺素在卵母细胞成熟过程中的作用：基因敲除小鼠的教训。

• 发表时间: 2010
• 期刊: J Mamm Ova Res 27
• 作者: Takahashi T;Igarashi H;Amita M;Hara S;Kurachi H
• 通讯作者: Kurachi H

マウス加齢卵における卵の質の改善を目的としたミトコンドリア移植の試み.

尝试通过线粒体移植来改善老年小鼠卵子的卵子质量。

• 发表时间: 2008
• 作者: 五十嵐秀樹;高橋俊文;網田光善;倉智博久
• 通讯作者: 倉智博久