The druggable approach using small nucleolar RNA of the neuropathic pain

使用小核仁 RNA 治疗神经性疼痛的药物方法

• 批准号: 20791070
• 负责人: NAKAE Aya
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791070/
• 关键词: snoRNA; RNA編集; 疼痛モデル; 核小体低分子RNA; セロトニン2C受容体; 眼窩下神経絞扼モデル; 選択的スプライシング; RBII-52; プラダー・ウィリ症候群; allodynia; 核小体RNA

The serotonin 2C receptor (5HTR2C) receives RNA editing. It is also known that as a result of alternative splicing of the 5HTR2C, a flame shift occurs and a non-functional receptor is created. Having studied the 5HTR2C RNA editing efficiency in a rat oro-facial neuropathic pain model (Nakae et al. EJN 2008), our interest turned to the factor contributing to RNA editing and efficacy of alternative splicing. In human study, HBII-52 (one kind of small nuclear RNA ; equal in function to RBII-52) contributes to regulating the 5HTR2C RNA editing and alternative splicing. In this research, an infra-orbital loose ligation model was used. The tendency of RBII-52 expression in injured and sham operated animals decreased. On the other hand, only the Va containing pattern (non-functional) increased in injured and sham rats. The relationship between RBII-52 expression, the 5HT2C-R alternative splicing and RNA editing was unclear. Modulation of 5HTR2C activity might be capable of far finer tuning than we previously imagined through noxious stimuli response, such as neuropathic pain.

5-羟色胺2C受体（5HTR2C）接收RNA编辑。还众所周知，由于5HTR2C的替代剪接，发生火焰转移并创建了非功能受体。在研究了大鼠异族神经性疼痛模型中的5HTR2C RNA编辑效率（Nakae等人EJN 2008）之后，我们的兴趣转向了促进RNA编辑和替代剪接功效的因素。在人类研究中，HBII-52（一种小核RNA；功能与RBII-52相等）有助于调节5HTR2C RNA编辑和替代剪接。在这项研究中，使用了眶下松动连接模型。 RBII-52在受伤和假手术动物中表达的趋势减少了。另一方面，受伤的大鼠和假大鼠中只有含有VA的模式（非功能）增加。 RBII-52表达，5HT2C-R替代剪接和RNA编辑之间的关系尚不清楚。 5HTR2C活性的调节可能比我们以前通过有害刺激反应（例如神经性疼痛）所想象的要精细的调整。

项目成果

眼窩下神経絞扼モデルにおける核小体RNA(RBII-52)の関わり

核仁 RNA (RBII-52) 参与眶下神经卡压模型

• 发表时间: 2009
• 作者: 中江文、真下節;他
• 通讯作者: 他

The snoRNA RBII-52 regulates alternative splicing of serotonin 2C receptor in the rat oro-facial neuropathic pain model.

snoRNA RBII-52 调节大鼠口面部神经病理性疼痛模型中血清素 2C 受体的选择性剪接。

• 期刊: Neuroscience 2009 abstract 15
• 作者: Aya Nakae;Kunihiro Nakai;Tatsuya Tanaka;Mari Yoshida;Akiko Mikami;Masaki Takashina;Satoshi Hagihira;Masahiko Shibata;Koichi Ueda;Takashi Mashimo.
• 通讯作者: Takashi Mashimo.

The role of snoRNA(RBII-52) in a rat model of oro-facial neuropathic pain

snoRNA(RBII-52)在大鼠口面部神经病理性疼痛模型中的作用

• 发表时间: 2009
• 期刊: European Journal of Anesthesiology 26(Supple45)
• 作者: A.Nakae;K.Nakai;他
• 通讯作者: 他

The role of snoRNA RBII-52 to the serotonin2C receptor in the rat oro-facial neuropathic pain model.

snoRNA RBII-52 在大鼠口面部神经病理性疼痛模型中对血清素 2C 受体的作用。

• 发表时间: 2009
• 期刊: Neuroscience (abstract)
• 作者: Kunihiro Nakai;Aya Nakae;Sosuke Oba;Masahiko Shibata;Takashi Mashimo;Koichi Ueda
• 通讯作者: Koichi Ueda

Recovery of motoneuron and locomotor function after chronic spinal cord injury depends on constitutive activity in 5-HT2C receptors.

慢性脊髓损伤后运动神经元和运动功能的恢复取决于 5-HT2C 受体的组成活性。

• 发表时间: 2010
• 期刊: Nature Medicine 26(10)
• 作者: Murray K;Nakae A;Stephens MJ;Rank M;D'Amico J;Harvey P;Li X;Harris L;Ballou EW;Anelli R;Heckman CJ;Mashimo T;Vavrek R;Sanelli L;Gorassini MA;Bennett DJ;Fouad K.
• 通讯作者: Fouad K.