The role of ALF4, a transcription factor, in the regulation of growth hormone secretion in the hypothalamus

转录因子 ALF4 在调节下丘脑生长激素分泌中的作用

• 批准号: 20790742
• 负责人: KOMORI Tadasuke
• 金额: $ 2.75万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790742/
• 关键词: 視床下部; 転写因子; グレリン; AMP-activated protein kinase; 摂食; 神床下部; 成長; 視床下部; 転写因子; グレリン; AMP-activated protein kinase; 摂食; 神床下部; 成長

AF5q31 expression was exclusively observed in growth hormone secretagogue-receptor (GHS-R)-expressing neurons during fasting. The intraperitoneal injection of ghrelin induced the expression of AF5q31 in the GHS-R-positive neurons of the arcuate nucleus and ventromedial hypothalamic nucleus. The decrease of AF5q31 expression in heterozygous mice attenuated the fasting-induced expression of AMPKα, as well as the expression of neuropeptide Y (NPY) and agouti-related peptide (AgRP) in the hypothalamus. Furthermore, the increase in fasting-induced food intake in heterozygous mice was less than that observed in wild-type mice. From these findings, ghrelin-induced AF5q31 in the hypothalamus appear to play an important role in the expression of AMPK, which may induce the expressions of NPY and AgRP, and thereby increase food intake.

在禁食过程中，AF5Q31的表达仅在生长型促分泌蛋白受体蛋白（GHS-R）表达神经元中观察到。腹膜内注射生长素蛋白诱导AF5Q31在弧形核us和腹膜下丘脑核心的GHS-R阳性神经元中的表达。杂合小鼠中AF5Q31表达的降低减轻了禁食诱导的AMPKα表达，以及下丘脑中神经肽Y（NPY）和与Agouti相关的肽（AGRP）的表达。此外，杂合小鼠禁食诱导的食物摄入量的增加小于野生型小鼠中观察到的食物。从这些发现中，下丘脑外观中诱导的AF5Q31在AMPK表达中起着重要作用，这可能会诱导NPY和AGRP的表达，从而增加食物摄入量。