A study for valorization and food safety of a Japanese brand chicken, the Satsuma-jidori, using DNA tests

使用 DNA 测试对日本品牌萨摩鸡进行价值评估和食品安全研究

• 批准号: 20780200
• 负责人: SHIMOGIRI Takeshi
• 金额: $ 2.75万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780200/
• 关键词: 育種; ゲノム; 畜産学; 遺伝学; 育種学; 動物; 育種; ゲノム; 畜産学; 遺伝学; 育種学; 動物

The Satsuma-jidori is a popular Japanese brand chicken, which crossbred Satsumadori and Rhode Island Red breeds. In this study, we developed a cost-effective and high-throughput chicken SNP genotyping system to discriminate the Satsuma-jidori from other chickens and conducted the following two studies. Study 1) To develop a cost-effective and rapid genomic DNA purification method in animals and birds, we examined the DNA purification method using glass fiber filter proven in plant genomics. Study 2) In order to identify the chicken breeds, we developed a cost-effective and high-throughput chicken SNP genotyping system using the DigiTag2 assay and applied this system to 89 birds from the five breeds including Satsumadori and Rhode Island Red. These results were as follows: 1) One μg of genomic DNA was purified from the chicken blood cell samples diluted 50-200 times. It could be used for ~5kb PCR amplification. 2) When the filter with the blood cells was stored for four weeks at room temperature, genomic DNA was purified. We purified the genomic DNAs using this method for 10 yen/sample and from 16 samples at 1 hour. 2) The DigiTag2 assay distinguished all 89 chickens examined here. The estimated probability of identity (P) was 8.00×10^<-20>. The P value is the probability that two individuals selected at random will have identical genotypes at all of the genotyped loci. Since the FAOSTAT database published in June 2009 showed that the total number of chickens raised worldwide in 2008 was approximately 1.84 ×10^<10> (http://faostat.fao.org/site/573/DesktopDefault.aspx?PageID=573#ancor), this SNP set would have power to identify all chickens in the world. Phylogenetic analysis distinguished all chickens and formed clusters of chickens belonging to the respective breeds. These results suggested that this SNP system may discriminate the chicken breeds.

Satsuma-jidori是一种流行的日本品牌鸡肉，它杂交了Satsumadori和Rhode Island Red Breeds。在这项研究中，我们开发了一种具有成本效益的高通量鸡SNP基因分型系统，以将Satsuma-Jidori与其他鸡区分开，并进行了以下两项研究。研究1）在动物和鸟类中开发一种具有成本效益和快速的基因组DNA纯化方法，我们使用植物基因组学证明的玻璃纤维过滤器检查了DNA纯化方法。研究2）为了鉴定鸡肉，我们使用Digitag2分析开发了一种经济高效且高通量的鸡SNP基因分型系统，并将该系统应用于包括Satsumadori和Rhode Island Red在内的五个品种的89只鸟类。这些结果如下：1）从稀释50-200次稀释的鸡血细胞样品中纯化了1μg基因组DNA。它可用于〜5KB PCR扩增。 2）当在室温下将带有血细胞的过滤器储存四个星期时，纯化了基因组DNA。我们使用此方法纯化了基因组DNA，以10日元/样品为1小时。 2）Digitag2分析区分了此处检查的所有89只鸡。身份（P）的估计概率为8.00×10^<-20>。 p值是在所有基因分型基因座中随机选择的两个个体将具有相同基因型的概率。自2009年6月发布的FAOSTAT数据库以来，2008年全球饲养的鸡的总数约为1.84×10^<10>（http://faostat.fao.org/site/573/573/desktopdefault.aspx?pageID=573#ancor）系统发育分析区分了属于各种品种的鸡的所有鸡和形成的簇。这些结果表明，该SNP系统可能会区分鸡肉品种。