Molecular Mechanisms of Establishment, Maintenance and Breakdown of Viral Persistent Infection

病毒持续感染建立、维持和分解的分子机制

• 批准号: 20590478
• 负责人: ITO Yasuhiko
• 金额: $ 3万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590478/
• 关键词: パラミクソウイルス; 持続感染; センダイウイルス; 細胞傷害; インターフェロン; 持続感染細胞; HN蛋白; F蛋白; パラインフルエンザイルス; 定量法; steady state; パラインフルエンザウイルス

We tried to devise a quantitative method for analyzing establishing-efficiency of persistent viral infection. Subsequently, we analyzed factors of Sendai virus contributing to persistent infection by applying the quantitative method, indicating that L protein plays a determinative role in establishment of and M protein has an additional ability to induce persistent infection Fpi protein also contributes to persistent viral infection, but HNpi protein shows suppressive effect on establishment of persistent viral infection. HPIV2 induced cell toxicity, that is, giant cell formation, was suppressed by Rho kinase inhibitor, indicating that Rho kinase plays a positive role in HPIV2 induced cell toxicity and C kinase inhibitor enhanced HPIV2 induced cell toxicity. Intriguingly, the protein kinases showed intracellular networks.

我们试图设计一种定量方法来分析持续病毒感染的建立效率。随后，我们应用定量方法分析了仙台病毒导致持续感染的因素，表明L蛋白在建立过程中起决定性作用，M蛋白具有诱导持续感染的附加能力。Fpi蛋白也有助于病毒持续感染，但HNpi 蛋白对持续病毒感染的建立具有抑制作用。 HPIV2诱导的细胞毒性，即巨细胞形成，被Rho激酶抑制剂抑制，表明Rho激酶在HPIV2诱导的细胞毒性中起积极作用，并且C激酶抑制剂增强HPIV2诱导的细胞毒性。有趣的是，蛋白激酶显示出细胞内网络。

项目成果

ムンプスワクチンの開発と開発過程における問題点

腮腺炎疫苗的研制及研制过程中存在的问题

• 发表时间: 2009
• 期刊: 小児感染免疫 21
• 作者: M.Ito;Y.Ito;et al.;Kouji Kobiyama;Kenichi Abe;小檜山康司;伊藤康彦
• 通讯作者: 伊藤康彦

Identification of domains on the fusion (f) protein trimer that influence the hemagglutinin-neunaminidase specificity of the f protein in mediatiing cell-cell fusion.

融合 (f) 蛋白三聚体上影响 f 蛋白在介导细胞-细胞融合中的血凝素-神经氨酸酶特异性的结构域的鉴定。

• 发表时间: 2011
• 期刊: J.Virology
• 作者: Tsurudome M;Ito M;Nishio M;Nakahashi M;Kawano M;Komada H;Nosaka T;Ito Y.
• 通讯作者: Ito Y.

Effects of multiple amino acids of the parainfluenza virus 5 fusion protein on its haemagglutinin-neuraminidase-independent fusion activity

• DOI: 10.1099/vir.0.006437-0
• 发表时间: 2009-02-01
• 期刊: JOURNAL OF GENERAL VIROLOGY
• 影响因子: 3.8
• 作者: Ito, Morihiro;Nishio, Machiko;Tsurudome, Masato
• 通讯作者: Tsurudome, Masato

Analysis of N-glycans in embryonated chicken egg chorioallantoic and amniotic cells responsible for binding and adaptation of human and avian influenza viruses.Sriwilaijaroen

对负责人类和禽流感病毒结合和适应的胚胎鸡蛋绒毛膜尿囊细胞和羊膜细胞中的 N-聚糖进行分析。Sriwilaijaroen

• 发表时间: 2009
• 期刊: Glycoconj J 26
• 作者: N.Sriwilaijaroen;Y.Ito;et al
• 通讯作者: et al

Spontaneous Formation of Multinucleated Giant Cells with Bone Resorbing Activity by Long Culture of Human Peripheral Blood CD14-positive Monocytes in vitro

人外周血CD14阳性单核细胞体外长培养自发形成具有骨吸收活性的多核巨细胞

• 发表时间: 2010
• 期刊: Cell Communication & Adhesion 17(1)
• 作者: Yuasa K;Avnet S;Salerno M;Mori K;Ishikawa H;Sudo A;Baldini N;Uchida A;Ito Y
• 通讯作者: Ito Y