Are rare earth elements essential for growth of methylotrophs?

稀土元素对于甲基营养菌的生长至关重要吗？

• 批准号: 20580075
• 负责人: KAWAI Keiichi
• 金额: $ 3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580075/
• 关键词: 希土類元素; メチロトローフ; メタノール脱水素酵素; Methylobacterium; Bradyrhizobium; xoxF; mxaF'; ランタン; セリウム; M.extorquens AM1; mxaF遺伝子; xoxF遺伝子; La; Ce; 誘導酵素; mxaF'遺伝子

Methanol dehydrogenase (MDH) of Methylobacterium sp.MAFF211642, a methyltrophic bacterium, was remarkably increased by addition of La to methanol medium, indicating that the MDH was induced by La.Induction of MDH by La was observed in Methylobacterium spp.MDH of Bradyrhizobium sp.MAFF 211645 was also induced by Ce.Thus, La or Ce can induce MDH in methylotrophic bacteria. Furthermore, molecular structure of both MDHs was homodimer that was different to the heterotetrameric structure of Ca-dependent MDH in Methylobacterium spp.In addition La-induced MDH in Methylobacterium spp.and Ce-induced MDH in Bradyrhizobium sp.MAFF211645 were found to be encoded by xoxF and mxaF', respectively. These phenomena were also observed in well-studied M.extorquens AM1.

Methanol dehydrogenase (MDH) of Methylobacterium sp.MAFF211642, a methyltrophic bacterium, was remarkably increased by addition of La to methanol medium, indicating that the MDH was induced by La.Induction of MDH by La was observed in Methylobacterium spp.MDH of Bradyrhizobium sp.MAFF 211645 was also induced by Ce.Thus, LA或CE可以诱导MDH中的MDH。此外，两种MDH的分子结构都是同二聚体，它与spp.中甲基甲基菌中Ca依赖性MDH的异次二二聚体结构不同，并在Bradyrhizobium sp.baff211645中添加了LA诱导的MDH，并在Bradyrhizobium sp.maft.mdh中诱导的MDH诱导了MDH。这些现象也在经过良好研究的M.Extorquens AM1中观察到。

项目成果

Methylobacterium sp.EU-1のメタノール脱水素酵素に及ぼす希土類元素の影響

稀土元素对甲基杆菌EU-1甲醇脱氢酶的影响

• 发表时间: 2008
• 作者: 奥田雅代;浅井健太郎;河合啓一;岩間智徳;鈴木徹
• 通讯作者: 鈴木徹

スカンジウム存在下でFusarium solani SC-2株により生産される色素

茄病镰孢菌株 SC-2 在钪存在下产生的色素

• 发表时间: 2010
• 作者: 成田千晴;坂尻裕子;岩間智徳;河合啓一
• 通讯作者: 河合啓一

レアメタル便覧,27章

稀有金属手册，第 27 章

• 发表时间: 2011
• 作者: Vu;H.T;et al.;河合啓一
• 通讯作者: 河合啓一

希土類の材料技術ハンドブック(足立吟也監修) 37章希土類の農業への応用

稀土材料技术手册（安达银也监修）第37章稀土在农业中的应用

• 发表时间: 2008
• 作者: Hoan Thi Vu;大塚重人;妹尾啓史;河合啓一
• 通讯作者: 河合啓一

Molecular structure of La^<3+>-induced methanol dehydrogenase-like protein in Methylobacterium radiotolerans

耐辐射甲基杆菌La^3诱导的甲醇脱氢酶样蛋白的分子结构

• 发表时间: 2011
• 期刊: Journal of Bioscience and Bioengineering
• 影响因子: 2.8
• 作者: Y.Hibi;H.Arafuka;M.Hamaji-ma T.lwama;K.Kawai
• 通讯作者: K.Kawai