The research of Electrochemotherapy and bone electroconductivity of the mirine osteosarcoma model of bone lesion.

小鼠骨肉瘤骨病变模型的电化学治疗及骨电导率研究。

• 批准号: 11671415
• 负责人: ISHIKAWA Akira
• 金额: $ 1.66万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671415/
• 关键词: osteosarcoma; in vivo electrochemotherapy; electroconductivity of bone; bleomycin; nude rat; drug concentration; histology; tomor necrosis; 骨肉腫; 骨電気伝導性; ヌードラット; bleomyciu; 腫瘍組織内濃度; 腫瘍壊死率; electrochemotherapy; SCIDマウス

INTRODUCTIONElectrochemotherapy is available to enhance the entry of anti-neoplastic agents into murine osteosarcoma cells in the bone lesion. In this experiment model, we investigated the efficacy and cause of electrochemotherapy whether it attributed to the electroconductivity of bone, the distance between electrode and the difference between configuration and number of electrode.MATERIALS AND METHODSTo produce solid murine tumors in bone, resected human osteosarcoma was cut into fine pieces, and inserted into the bone marrow of the hind-limb of Nude rat converting from the SCID mouse.After transplantation, the tumor diameter was checked by X-ray photogram and the experiment was started when it reached 5 mm. The electroporation was done by the electroporator (T820) of BTX.The changes of tumor volume were compared sequentially among the two groups : Drug (D) + Electroporation (E) and neither D nor E.As an anti-neoplastic agent, we used bleomycin (BLM).The electrical resistivity betwee … More n electrodes which held the tumor was measured, and heat (Calaory) which arises by electroporation was calculated.The tumor tissue concentration of BLM was analyzed based on the bio-assay and compared among the two groups : D+E and D without E.Histology was investigated on the tumor necrosis.RESULTS AND DISCUSSIONSThere was the reduction of the tumor diameter in the electroporation (E) group. The reduction of the tumor diameter has been obtained in the group in which the distance between electrodes spread 10mm, 15mm, 20mm and interval. Effective examples were the 23 limbs in 38 limbs.The effective example increased in multiple dose group under operation, when it was compared at the frequency in which 1 time operation, 2 time operation, 3 time operation did (D+E+).The concentration in the tumor tissue of BLM showed the high value, since it has it, in comparing without electroporation group (D+E-) in anti-cancer drug and electroporation group (D+E+), and it seemed to be the effect of electroporation that BLM was incorporated in the interosseous tumor tissue. The production heat calculated from electrical resistivity between tumors did not bring about the effect in anti-tumor effect. In-the example of showing the progress that the bone destruction was restored in X-ray photogram, there were necrosis and elimination of the tumor cell in the pathohistological retrieval. It seemed to be the opinion which proved that electrochemotherapy for the interosseous lesion through the bone has the cytotoxicity reaction.The significance could not be accepted between distance and effect between electrodes. Less

简介电化学疗法可增强抗肿瘤药物进入骨病灶中的小鼠骨肉瘤细胞。在该实验模型中，我们研究了电化学疗法的效果和原因是否归因于骨的导电性、电极之间的距离和差异。电极的配置和数量之间存在差异。 材料和方法为了在骨中产生实体鼠肿瘤，将切除的人骨肉瘤切成细片，并插入骨髓中SCID小鼠转化的裸鼠后肢。移植后，通过X射线照片检查肿瘤直径，当肿瘤直径达到5mm时开始实验，使用BTX电穿孔仪（T820）进行电穿孔。 .依次比较两组肿瘤体积的变化：药物（D）+电穿孔（E），既不D也不E。作为抗肿瘤剂，我们使用博来霉素(BLM)。测量固定肿瘤的n个电极之间的电阻率，并计算电穿孔产生的热量(卡路里)。基于生物测定分析BLM的肿瘤组织浓度，并比较两者结果与讨论电穿孔(E)组肿瘤直径缩小。在电极间距分别为10mm、15mm、20mm和间隔的组中，38条肢体中的有效例为23条。在手术中的多剂量组中，以频率进行比较时，有效例有所增加。 1次手术、2次手术、3次手术（D+E+）。与未进行电穿孔的组相比，肿瘤组织中的浓度显示出高值，因为有它。抗癌药物和电穿孔组(D+E+)中的(D+E-)，并且BLM被纳入骨间肿瘤组织中，这似乎是电穿孔的效果，根据肿瘤之间的电阻率计算的产热没有。带来抗肿瘤作用-在X射线照片中显示骨质破坏恢复进展的例子中，在病理组织学检索中似乎有肿瘤细胞的坏死和消除。证明通过骨对骨间病变进行电化学治疗具有细胞毒性反应的观点。电极之间的距离和效果之间的意义不能被接受。

项目成果

AKIRA ISHIKAWA: "Low-Voltage Electrochemotherapy to Murine Osteosarcoma Model"The Journal of the Japanese Orthopaedic Association. Vol.74. S1252 (2000)

AKIRA ISHIKAWA：“低电压电化学疗法对小鼠骨肉瘤模型”日本骨科协会杂志。

AKIRA ISHIKAWA: "Electrochemotherapy to the Bone Lesion of Murine Osteosarcoma"The Journal of the Japanese Orthopaedic Association. Vol.73. S1227 (1999)

AKIRA ISHIKAWA：“电化学疗法治疗小鼠骨肉瘤骨病变”日本骨科协会杂志。

石川 朗: "マウス骨肉腫の骨内病変に対するElectrochemotherapyの有効性"日本整形外科学会雑誌. 73. S1227 (1999)

Akira Ishikawa：“电化学疗法对小鼠骨肉瘤骨内病变的疗效”日本骨科学会杂志 73. S1227 (1999)。

Ishikawa A: "Electrochemotherapy effect of bleomycin to the murine osteosarcoma."Sosiete International de Recherche en Orthopedie et Traumatologie(SIROT). 8. 733-736 (1999)

Ishikawa A：“博来霉素对小鼠骨肉瘤的电化学治疗效果。”Sosiete International de Recherche en Orthopedie et Traumatologie (SIROT)。

石川 朗: "マウス骨肉腫に対する低電圧Electrochemotherapyの有効性"日本整形外科学会雑誌. 74. S1252 (2000)

Akira Ishikawa：“低压电化学疗法对小鼠骨肉瘤的疗效”，日本骨科学会杂志 74. S1252 (2000)。