Immortalization of prion protein deficient cell lines and establishment of prion-susceptible susceptible cell lines by introduction of prion protein genes.

朊病毒蛋白缺陷细胞系的永生化和通过引入朊病毒蛋白基因建立朊病毒敏感的易感细胞系。

• 批准号: 20380166
• 负责人: ONODERA Takashi
• 金额: $ 12.15万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380166/
• 关键词: 免疫; ウイルス; プリオン; 感染症; マクロファージ; 細胞不死化; BSE; スクレイピー; プリオン病; プリオン遺伝子欠損; プリオン遺伝子欠損マウス; 不死化遺伝子; マウス; 骨髄細胞; 免疫; ウイルス; プリオン; 感染症; マクロファージ; 細胞不死化; BSE; スクレイピー; プリオン病; プリオン遺伝子欠損; プリオン遺伝子欠損マウス; 不死化遺伝子; マウス; 骨髄細胞

After prion infection, an abnormal isoform of prion protein (PrP (Sc)) converts the cellular isoform of prion protein (PrP (C)) into PrP(Sc). PrP (C)-to-PrP (Sc) conversion leads to PrP (Sc) accumulation and PrP (C) deficiency, contributing etiologically to induction of prion diseases. Presently, most of the diagnostic methods for prion diseases are dependent on PrP (Sc) detection. Highly sensitive/accurate specific detection of PrP (Sc) in many different samples is a prerequisite for attempts to develop reliable detection methods. Towards this goal, several methods have recently been developed to facilitate sensitive and precise detection of PrP (Sc), namely, protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, capillary gel electrophoresis, fluorescence correlation spectroscopy, flow microbead immunoassay, etc. Additionally, functionally relevant prion-susceptible cell culture models that recognize the complexity of the mechanisms of prion infection have also been pursued, not only in relation to diagnosis, but also in relation to prion biology. Prion protein (PrP) gene-deficient neuronal cell lines that can clearly elucidate PrP (C) functions would contribute to understanding of the prion infection mechanism. In this review, we describe the trend in recent development of diagnostic methods and cell culture models for prion diseases and their potential applications in prion biology.

prion感染后，prion蛋白（PRP（SC））的异常同工型将prion蛋白（PRP（C））的细胞同工型转换为PRP（SC）。 PRP（C）-TO-PRP（SC）转化导致PRP（SC）积累和PRP（C）缺乏症，从病因上促进了prion疾病。目前，大多数有关prion疾病的诊断方法取决于PRP（SC）检测。在许多不同样本中，PRP（SC）的高度敏感/准确的特异性检测是尝试开发可靠检测方法的先决条件。为了实现这一目标，最近已经开发了几种方法来促进PRP（SC）的敏感和精确检测，即蛋白质错误折叠环状扩增，构型依赖性免疫测定，分离型植物型灯膜荧光荧光测定，毛细血管免疫电池，荧光电池相关，荧光相关镜，荧光镜，荧光相关型相关型相关型，荧光相关型相关镜，且功能相关型相关型，荧光镜，相关的镜头，还追求了识别prion感染机制复杂性的可敏感的细胞培养模型，不仅与诊断有关，而且与prion生物学有关。可以清楚地阐明PRP（C）功能的PRION蛋白（PRP）基因缺陷型神经元细胞系将有助于理解Prion感染机制。在这篇综述中，我们描述了诊断方法和细胞培养模型的近期发展趋势，用于prion疾病及其在prion生物学中的潜在应用。