DNA damage and modification of immunity by low frequency magnetic fields

低频磁场对 DNA 的损伤和免疫力的改变

• 批准号: 20200024
• 负责人: MIYATA Hidetake
• 金额: $ 18.72万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20200024/
• 关键词: 微量化学物質汚染評価; HUVEC; 一酸化窒素; DAF-2; 50ヘルツ磁場; ジアミノフルオレセイン; 血管内皮細胞; 変動磁場; 細胞参加ストレス

To investigate the effect of magnetic fields on the cell, human umbilical vein endothelial cells (HUVECs) were exposed to a 50-Hz, 1-mT sinusoidal magnetic field. Nitric oxide (NO) production by HUVEC was estimated from the fluorescence of DAF-2, an NO indicator. The NO production of exposed and unexposed (sham) cells exhibitde statistically significant difference. This finding is consistent with previous studies in that low frequency magnetic fields affects NO production; however, our results demonstrate that in some cases, NO production was higher for the exposed group than the sham group, while in other cases the reverse was true. We suggest that the response of HUVECs to magnetic field was dependent on the condition of the cell eg., its stage in the cell cycle.

为了研究磁场对细胞的影响，将人脐静脉内皮细胞 (HUVEC) 暴露于 50 Hz、1 mT 正弦磁场。 HUVEC 产生的一氧化氮 (NO) 是根据 NO 指示剂 DAF-2 的荧光估算的。暴露和未暴露（假）细胞的NO产生表现出统计学显着差异。这一发现与之前的研究一致，即低频磁场会影响 NO 的产生；然而，我们的结果表明，在某些情况下，暴露组的 NO 产量高于假手术组，而在其他情况下，情况恰恰相反。我们认为 HUVEC 对磁场的反应取决于细胞的状况，例如其在细胞周期中的阶段。

项目成果

Clinicopathological Features of Malignant Lymphoma in Japan: The Miyagi Study

• DOI: 10.1620/tjem.224.151
• 发表时间: 2011-06-01
• 期刊: TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
• 影响因子: 2.2
• 作者: Miura, Yukiko;Fukuhara, Noriko;Harigae, Hideo
• 通讯作者: Harigae, Hideo

50Hz磁場曝露によるHUVEC(ヒト臍帯血管内皮細胞)のNO産生

暴露于 50Hz 磁场后 HUVEC（人脐带血管内皮细胞）中 NO 的产生

• 发表时间: 2010
• 作者: 顔暢子;本堂毅;宮田英威
• 通讯作者: 宮田英威

Allogeneic hematopoietic stem cell transplant following chemotherapy containing 1-asparaginase as a promising treatment for patients with relapsed or refractory extranodal natural killer/T cell lymphoma, nasal type

含 1-天冬酰胺酶化疗后的同种异体造血干细胞移植是治疗复发性或难治性结外自然杀伤/T 细胞淋巴瘤（鼻型）患者的一种有希望的治疗方法

• 发表时间: 2010
• 期刊: Leuk Lymphoma
• 作者: Yokoyama H;Yamamoto H;Tohmiya Y;Yamada MF;Ohguchi H;Ohnishi Y;Okitsu Y;Fukuhara N;Ohba-Ohtsuka R;Kohata K;Ishizawa K;Kameoka J;Harigae H
• 通讯作者: Harigae H

Rhythmic behavior of social insects from single to multibody

• DOI: 10.1016/j.robot.2011.06.016
• 发表时间: 2012-05-01
• 期刊: ROBOTICS AND AUTONOMOUS SYSTEMS
• 影响因子: 4.3
• 作者: Hayashi, Yoshikatsu;Yuki, Mai;Tsuji, Kazuki
• 通讯作者: Tsuji, Kazuki

The effect of a 50-Hz sinusoidal magnetic field on nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs)

50 Hz 正弦磁场对人脐静脉内皮细胞 (HUVEC) 产生一氧化氮 (NO) 的影响

• 发表时间: 2012
• 期刊: J Phys Conf Ser
• 作者: Miyata H;Ishizawa K;Ishido M;Sugawara K;Murase M;Hondou T
• 通讯作者: Hondou T