Development of a novel one-step procedure for articular cartilage repair using minced cartilage and mesenchymal stem

开发一种使用碎软骨和间充质干进行关节软骨修复的新型一步式手术

• 批准号: 19591750
• 负责人: NAKAGAWA Koichi
• 金额: $ 2.33万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591750/
• 关键词: 関節病学; 関節軟骨修復; 滑膜細胞; 細切軟骨; 移植; 幹細胞; 軟骨分化; 脂肪細胞; 軟骨細胞; 共培養

[Objective] To develop a novel implantation procedure without in vitro culture, the effect of co-implantation of minced cartilage and synovial cells on the chondrogenic differentiation of the synovial tissue-derived mesenchymal stem cells was investigated.[Methods] Rabbit synovial cells were isolated by enzyme treatment for 1h and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group), both minced articular cartilage and synovial cells (AC+SC group) or neither cartilage nor synovial cells (NC group) were implanted subcutaneously in nude mice and then examined the histological and biochemical findings.[Results] Safranin-O staining tended to be stronger, and proteoglycan content (per weight) was significantly increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were significantly enhanced in the AC+SC group.[Conclusion] Co-implantation of minced cartilage with synovial cells enhances chondrogenic differentiation. The use of implant grafts containing both minced cartilage and synovial cells may be a novel approach for one-step joint cartilage repair.

[目的] 开发一种无需体外培养的新型植入方法，探讨碎软骨与滑膜细胞共植入对滑膜组织源性间充质干细胞成软骨分化的影响。 [方法] 分离兔滑膜细胞酶处理1h，包埋于纤维蛋白凝胶中。将仅含有滑膜细胞（SC组）、同时含有碎关节软骨和滑膜细胞（AC+SC组）或既不含软骨又不含滑膜细胞（NC组）的纤维蛋白凝胶植入物皮下植入裸鼠，然后检查组织学和生化结果[结果]与SC组相比，AC+SC组的番红-O染色趋于更强，并且蛋白多糖含量（单位重量）显着增加。此外，AC+SC组II型胶原和Sox9表达显着增强。[结论]碎软骨与滑膜细胞共植入可增强软骨分化。使用含有碎软骨和滑膜细胞的植入物移植物可能是一种一步式关节软骨修复的新方法。

项目成果

細切軟骨片との共存移植が滑膜細胞の軟骨分化に与える影響

软骨碎片联合移植对滑膜细胞软骨分化的影响

• 发表时间: 2009
• 期刊: 千葉スポーツ医学研究会雑誌 6
• 作者: ファロウ・ルアイ、中川晃一;ほか
• 通讯作者: ほか

Effect of platelet-rich plasma gel on rabbit articular chondrocytes cultured in alginate beads

富血小板血浆凝胶对海藻酸盐珠培养的兔关节软骨细胞的影响

• 发表时间: 2009
• 作者: Fallouh L;Nakagawa K;Sasho T;Arai M;Kitahara S;Wada Y;Moriya H;Takahashi K
• 通讯作者: Takahashi K

関節軟骨細胞との共培養が滑膜細胞の軟骨分化に与える影響の解析

与关节软骨细胞共培养对滑膜细胞软骨分化的影响分析

• 发表时间: 2008
• 作者: 守屋拓朗;佐粧孝久;中川晃一;北原聡太;鶴岡弘章;荒井桃子;高橋和久;和田佑一;山下剛司;守屋秀繁
• 通讯作者: 守屋秀繁

滑膜細胞および細切軟骨片の軟骨修復における利用

滑膜细胞和软骨碎片在软骨修复中的应用

• 发表时间: 2009

滑膜細胞を用いた軟骨再生技術の開発

开发利用滑膜细胞的软骨再生技术

• 发表时间: 2007
• 作者: 守屋 拓朗;中川 晃一;ほか
• 通讯作者: ほか