Effect of nitric oxide on release of neurotransmitters in the brain

一氧化氮对大脑神经递质释放的影响

基本信息

批准号：
10670917
负责人：
SUZUKI Eiji
金额：
$ 2.05万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670917/
关键词：
nitric oxide immobilization stress nitric oxide synthase nitrite nitrate brain rat うつ病

项目摘要

At first, we investigated whether nitric oxide synthase (NOS) gene was induced by immobilization stress (IMS) in rat hypothalamus, hippocampus, cerebral cortex, and cerebellum, using reverse transcription-polymerase chain reaction. Animals were treated in accordance with the National Institutes of Health guide for the care and use of Laboratory animals. Immobilization stress induced the expression of iNOS mRNA in every brain region examined with a peak around 30 or 60 min. This result suggests that, since iNOS is tanscriptionally-regulated enzyme, it is induced by stress exposure in the brain.The objection of the second experiment was that we investigated whether nitric oxide (NO) levels changed after the initiation of IMS in the hypothalamus of free-moving rats, Rats were anesthetized and inplanted a guide canule in the hypothalamus. After 1 week, a microdialysis probe was inserted into the hypothalamus and was perfused with Hanks's solution. The dialysate were injected in an automate … More d NO detector-hyperformance-liquid-chromatography system (ENO-10, Eicom, Kyoto, Japan) directly. Four or more hours later, assays of NOィイD3-(/)2ィエD3 and NOィイD3-(/)3ィエD3 levels were started, and then the basal levels were measured for two or more hours. Between 40 and 120 min after starting of iMS, NOィイD3-(/)2ィエD3 and NOィイD3-(/)3ィエD3 levels increased significantly.Finally, we investigated plasma NOィイD3-(/)2ィエD3 and NOィイD3-(/)3ィエD3 levels in untreated 17 patients with depressive episodes, untreated seven patients with anxiety disorders, and 12 healthy controls. Written informed consent was obtained from each individual.No significant difference was found in plasma nitrite levels among the groups. Plasma NOィイD3-(/)3ィエD3 levels in depressed patients were significantly higher than those in controls or patients with anxiety disorders (Scheffe's F test, P<0.05, figure). Moreover, in patients with depressive episodes, nitrate levels significantly decreased, approximately to control levels, after recovery (Wilcoxon signed-ranks test, P<0.01, figure).Unclear remains the source of the surplus production of nitric oxide in patients with depressive episodes. However, measurement of plasma nitrate levels might be useful in the differential diagnosis of affective disorders versus anxiety disorders. Less

首先，我们使用逆转录聚合酶链式反应研究了大鼠下丘脑、海马、大脑皮层和小脑中一氧化氮合酶（NOS）基因是否由固定应激（IMS）诱导。动物按照国立研究所的方法进行处理。实验动物护理和使用的健康指南固定应激诱导每个大脑区域的 iNOS mRNA 表达在 30 或 60 分钟左右达到峰值。表明，由于 iNOS 是转录调节酶，它是由大脑中的压力暴露诱导的。第二个实验的反对意见是，我们研究了在 IMS 启动后，自由组下丘脑中一氧化氮 (NO) 水平是否发生变化。移动大鼠，将大鼠麻醉并在下丘脑植入引导管。1周后，将微透析探针插入下丘脑并用汉克斯溶液灌注。四小时或更长时间后，将透析液直接注入自动 D NO 检测器高效液相色谱系统（ENO-10，Eicom，京都，日本）中，进行 NOiD3-(/)2ie D3 和 D3-( 的测定。 /)开始3D3水平，然后在开始后40至120分钟之间测量基础水平。 iMS、NOI D3-(/)2 D3 和 NO D3-(/)3 D3 水平显着升高。最后，我们调查了 17 名未经治疗的 17 名患有糖尿病的患者的血浆 NOI D3-(/)2 D3 和 NO D3-(/)3D3 水平。抑郁发作、未经治疗的 7 名焦虑症患者和 12 名健康对照者均获得了书面知情同意书。血浆中未发现显着差异。抑郁症患者的血浆亚硝酸盐水平显着高于对照组或焦虑症患者（Scheffe's F检验，P<0.05，图），抑郁症患者的硝酸盐水平显着下降，接近于对照水平。恢复后（Wilcoxon 符号秩检验，P<0.01，图）。硝酸过剩生产的来源仍不清楚然而，测量血浆硝酸盐水平可能有助于情感障碍与焦虑症的鉴别诊断。