Elusidation of the enhancing factor in paraquat toxicity under magnesium restriction

镁限制下百草枯毒性增强因素的阐明

基本信息

批准号：
10670385
负责人：
MINAKATA Kayoko
金额：
$ 2.24万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670385/
关键词：
paraquat magnesium copper iron zinc calcium reactive oxygen atomic absorption spectrometry マンガン ESR ラジカルジクワット活性酵素抗酸化剤ビタミンC 電子スピン共鳴

项目摘要

A highly sensitive but simple method for the quantification of Cu was devised (published in Clin Chem, 2001, JpnJ Forensic Toxicol, 2001). Only 5μl of plasma or 1.5 ml of running water is enough to quantify Cu. By use of this method tissue Cu levels of rats intoxicated with paraquat at 250 ppm in the diet were examined. The contents of Fe, Mg and Zn were determined by atomic absorption spectrometry, Shimadsu AA-6200 which was obtained by the present Grant-in-Aid. In intoxicated rats, Cu increased 2-fold of the control in lung, liver or plasma, and decreased to 0.5-foldin kidney. Fe increased 2-fold in liver or spleen, and decreased to 0.5-fold in plasma. Mg increased 2-fold in kidney. Liver Mg or Zn increased significantly (J Toxicol Environ Health, 2002)Mg restricted rats dosed with 125 ppm paraquat gave the same changes in these minerals (JpnJ Legal Med, 2001). However, Ca in kidney increased 10-foldin Mg restricted rats (will be reportedinThe86th Congress ofJpn Soc Legal Med, 2002). Usually it takes more than 35days to accumulate Ca at such a high level by dietary Mg restriction only. Feeding paraquat at 125 ppm under Mg restriction accelerated both paraquat toxicosis and Mg deficiency. The kidney Ca of rats fed with 125 ppm paraquat under all mineral restriction increased to 20-fold, where the ratio of Ca to Mg in the diet was maintained (will be reported in ISALM, 2002).

设计了一种高度灵敏但简单的 Cu 定量方法（发表于 Clin Chem，2001 年，JpnJ Forensic Toxicol，2001 年），使用该方法只需 5μl 血浆或 1.5 ml 自来水即可定量 Cu。检测了饮食中 250 ppm 百草枯中毒大鼠的 Cu 含量，并采用原子法测定了 Fe、Mg 和 Zn 的含量。吸收光谱法，Shimadsu AA-6200，由目前的 Grant-in-Aid 获得，在中毒大鼠中，肺、肝脏或血浆中的铜含量增加了 2 倍，而肾脏中的铁含量增加了 2 倍。肝脏或脾脏中镁含量降低至 0.5 倍，肾脏中镁含量增加 2 倍（J Toxicol Environ Health， 2002) 服用 125 ppm 百草枯的 Mg 大鼠在这些受限制的矿物质中产生了相同的变化（JpnJ Legal Med, 2001），但是，在 Mg 受限制的大鼠中，肾脏中的 Ca 增加了 10 倍（将在第 86 届 Jpn Soc Legal Med 大会上报告，2002）。通常仅通过饮食限制镁的摄入需要 35 天以上才能积累如此高的钙水平。镁限制下的 125 ppm 会加速百草枯中毒和镁缺乏在所有矿物质限制下喂食 125 ppm 百草枯的大鼠，其肾钙增加至 20 倍，其中钙与镁的比例保持不变（将在伊萨姆，2002）。