Fundamental Study of Surface Plasmon Resonance and Analysis of Biomolecular based on the Antigen-antibody Reactions

基于抗原抗体反应的表面等离子共振和生物分子分析的基础研究

基本信息

批准号：
10650800
负责人：
KAMATA Satsuo
金额：
$ 1.92万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650800/
关键词：
Surface plasmon resonance sensor Thin gold film ABO blood type Reaction of antigen-antibody coupling Saliva Bloodstain Tumor antigen α-fetoprotein (AFP)Pesticide 表面プラズモン共鳴金属薄膜 ABO血液型簡易測定尿塩化ベンゼトニウム

项目摘要

The surface plasmon resonance (SPR) method is a powerful tool for determining the optical properties of thin dielectric layers. In the SPR method, the angular dependence of the reflectivity from a dielectric surface (gold film) is studied in the region to total internal reflections. Surface plasmon is a surface charge-density wave at a metal surfaces attached sample. The evanesent, wave is produced to the interface between the prism and slide glass coated with thin gold film. At critical angle, surface plasmon is exited by resonance with evanesent, wave and then the reflectivity is decrease sensitively. The resonance angle is very sensitive to variations in the refractive index of the medium just outside the metal thin film. So we measured the refractive index of the biomolecules to be analyze by the SPR method. In this project, we investigated first the effect of SPR response for the thickness change of metal film and the flatness which was observed by AFM. Under the appropriate condition, we studied the analysis of some biomolecules as follows.l) ABO blood type of bloodstain and saliva were measured by the reaction of monoclonal antibody with antigen sample on the thin gold film rapidly and sensitively. 2) The tumor antigen, α-fetoprotein (AFP), in serum was analyzed sensitively and rapidly in the region of 1 IU/ml to 20 IU/ml. 3) The gold-coated unclad optical fiber responded rapidly to the ethanol and ethylene glycol by the SPR mechanism.

表面等离子体共振 (SPR) 方法是确定薄介电层光学特性的有力工具。在 SPR 方法中，研究了全内反射区域中介电表面（金膜）反射率的角度依赖性。表面等离子体激元是附着在样品上的金属表面上的表面电荷密度波，在棱镜和涂有薄金膜的载玻片之间的界面上产生表面等离子体激元。通过与消失波共振而激发，然后反射率敏感地降低，共振角对金属薄膜外部介质的折射率的变化非常敏感，因此我们测量了待分析的生物分子的折射率。在本项目中，我们首先研究了SPR响应对AFM观察到的金属膜厚度变化和平整度的影响，我们研究了以下一些生物分子的分析。通过单克隆抗体与金薄膜上的抗原样本反应，快速、灵敏地测定血迹和唾液的ABO血型。2)在该区域灵敏、快速地分析血清中的肿瘤抗原甲胎蛋白(AFP)。 1 IU/ml 至 20 IU/ml 3) 镀金裸光纤通过 SPR 机制对乙醇和乙二醇做出快速响应。