Structure and properties of amylopectin isolated from endosperm starch of low-amylose bread wheat

低直链淀粉面包小麦胚乳淀粉支链淀粉的结构与性质

• 批准号: 19500673
• 负责人: YASUI Takeshi
• 金额: $ 2.91万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19500673/
• 关键词: 食素材; 澱粉; 食品; 植物

Large A-type endosperm starch granules were isolated from near-isogenic waxy and non-waxy lines and low-amylose mutant lines of bread wheat with a common genetic background. The amylose contents of A-type starch ranged from 2.6% to 23.6%. Amylopectin was isolated by concanavalin A (Con A) precipitation from the isolated starch. The λ_<max> and blue values at 680 nm of the iodine-amylopectin complex were not significantly different among the isolated amylopectins, indicating that amylopectins from non-waxy and low-amylose lines did not contain such long chains as amylose or extra-long chains of amylopectin affecting iodine complex properties. Chain-length distribution profiles measured by both high-performance size exclusion chromatography (HPSEC) and high-performance anion-exchange chromatography (HPAEC) showed that the amylopectin structure of these lines was indistinguishable from each other. Extra-long chains were not detected in the amylopectins by HPSEC measurement. The alleles on the Wx-D1 locus, i.e. Wx-D1a, Wx-D1d, Wx-D1f, and Wx-D1g, responsible for granule-bound starch synthase (GBSS I) biosynthesis had no influence on the properties of iodine-amylopectin complex and the chain-length distribution profiles of amylopectin.

大型A型内生植物是从近乎同等的蜡和蜡状蜡中分离出来的，低淀粉突变型的Breat线具有共同的网络背景。 （CON A）来自分离的淀粉的沉淀。由高性能尺寸排除色谱法（HPSEC）AD高性能阴离子 - 交换色谱（HPAEC）均可表明，这些线的链球菌结构与彼此没有区别。 D1基因座，即WX-D1A，WX-D1D，WX-D1F和WX-D1G，负责颗粒结合的淀粉合酶（GBSS I）生物合成没有影响淀粉蛋白的。

项目成果

Chain-length Distribution Profiles of Amylopectin Isolated from Endosperm Starch of Waxy and Low-amylose Bread Wheat (Triticum aestivum L.) Lines with Common Genetic Background

• DOI: 10.1002/star.200900177
• 发表时间: 2009-12-01
• 期刊: STARCH-STARKE
• 影响因子: 2.3
• 作者: Yasui, Takeshi;Ashida, Kanae;Sasaki, Tomoko
• 通讯作者: Sasaki, Tomoko

Physicochemical characteristics of starches of two sets of near-isogenic wheat lines with different amylose content

两组不同直链淀粉含量的近等基因小麦品系淀粉理化特性

• 发表时间: 2008
• 期刊: Starch/Starke 60
• 作者: Pham Van Hung;Takeshi Yasui;Tomoko Maeda;Naofumi Morita
• 通讯作者: Naofumi Morita