Molecular mechanisms on differentiation or function of osteoblasts and osteoclasts by the Wnt/LRP signaling pathway

Wnt/LRP信号通路影响成骨细胞和破骨细胞分化或功能的分子机制

• 批准号: 19390469
• 负责人: TAMURA Masato
• 金额: $ 12.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390469/
• 关键词: 骨形成; 骨吸収; Wntシグナル; 骨芽細胞; Wnt; BMP; オステオプロテゲリン; miRNA; 骨形成; 骨吸収; Wntシグナル; 骨芽細胞; Wnt; BMP; オステオプロテゲリン; miRNA

In this study, we demonstrated that two cellular signaling pathways of Wnt and bone morphogenetic protein (BMP) control both differentiation and function of osteoblasts and osteoclasts during processes of bone formation and resorption. These Wnt and BMP signaling regulates osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor, which can inhibit the production of osteoclasts. The regulation of OPG expression is mediated through two transcription pathways such as Wnt and BMP that involve the OPG promoter. We also demonstrated that osteoblastic differentiation mediated by certain miRNA expression and BMP-2 down-regulates miR-206 expression at the post-transcriptional level and that BMP-2 could regulate miRNA biogenesis by a novel mechanism.

在这项研究中，我们证明了Wnt和骨形态发生蛋白（BMP）的两个细胞信号通路控制骨形成和吸收过程中成骨细胞和破骨细胞的分化和功能。这些Wnt和BMP信号传导调节骨蛋白蛋白蛋白（OPG），也称为破骨细胞生成因子，可以抑制破骨细胞的产生。 OPG表达的调节是通过涉及OPG启动子的两个转录途径（例如Wnt和BMP）介导的。我们还证明，由某些miRNA表达和BMP-2介导的成骨细胞分化在转录后水平下降了miR-206表达，并且BMP-2可以通过一种新机制来调节miRNA生物发生。