Utilization of protoplast for producing and screening of useful metabolites and it's application.

利用原生质体产生和筛选有用代谢物及其应用。

• 批准号: 19360370
• 负责人: AOYAGI Hideki
• 金额: $ 11.98万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19360370/
• 关键词: 微生物プロトプラスト; 植物プロトプラスト; 人工細胞壁; 細胞壁成分; タキソール; メタボロミクス; バイオリアクター; 有用代謝産物; ナノバブル; バイオリアクーダニー; 微生物プロトプラスト; 植物プロトプラスト; 人工細胞壁; 細胞壁成分; タキソール; メタボロミクス; バイオリアクター; 有用代謝産物; ナノバブル; バイオリアクーダニー

A high-speed system for production of useful materials using protoplasts was developed. In microbial and plant protoplasts, some products are released freely into the broth with the double consequences of increasing overall productivity and facilitating down stream processing. Furthermore, since many high molecular weight substances which are otherwise not accessible to the cells (due to the presence of cell wall) can directly access the cell membrane in protoplasts, the composition and concentrations of the metabolites produced by protoplasts vary from those produced by cells. It was found that the yeast protoplasts are cultured in a liquid static culture so as to diffuse cell wall components in the culture broth with the result that while maintaining the state of the protoplasts, protoplasts could produce the cell wall components in the culture broth. By optimizing the culture conditions, large amount of the cell wall components with antitumor activity was produced. Taxus protoplasts with artificial cell walls produce taxiods efficiently and released the compounds into the medium. Moreover, the adequate supply of methyl-jasmonate, etc. were useful to enhance the taxoids production in Taxus protoplasts. This novel system has great potential in useful metabolite production.

开发了一种使用原生质体生产有用材料的高速系统。在微生物和植物原生质体中，某些产品被自由释放到肉汤中，其两倍后果是提高整体生产率并促进下水道加工。此外，由于许多细胞无法进入的高分子量物质（由于细胞壁的存在）可以直接访问原生质体中的细胞膜，因此原生质体产生的代谢产物的组成和浓度与细胞产生的代谢产物不同。发现酵母原生质体是在液态静态培养物中培养的，以便在培养汤中扩散细胞壁成分，结果是在维持原生质体的状态时，原生质体可以在培养基中产生细胞壁成分。通过优化培养条件，产生了大量具有抗肿瘤活性的细胞壁成分。带有人造细胞壁的出租车原生物体有效地产生出租车，并将化合物释放到培养基中。此外，足够的甲基 - 贾斯酯等供应对于增强出租车原生质体中的紫类动物的产生非常有用。这个新型系统在有用的代谢产物中具有巨大的潜力。