Enhancement of Maturation Using Low-intensity Pulsed Ultrasound on Bone Formation Induced by rhGDF-5

使用低强度脉冲超声促进 rhGDF-5 诱导的骨形成的成熟

基本信息

批准号：
18592137
负责人：
HIROSE Yukito
金额：
$ 2.62万
依托单位：
Health Sciences University of Hokkaido
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592137/
关键词：
Regenerative Medicine dentistry stress bioactivity bone formation bone density LIPUs rhGDF-5 rh-GDF-5

项目摘要

In our previous study, we were able to induce bone formation by using rhGDF-5. However, the results of that study showed that even though bone formation occurred, the resulting bone growth was immature. This study assessed the efficacy of the low-intensity pulsed ultrasound stimulation (LIPUs)to enhance the bone maturation that was induced by the recombinant human growth differentiation factor 5 (rhGDF-5). This study used adult beagle dogs that weighed about 12 kg. The composite of β-TCP matrix with rhGDF-5 is made of rhGDF-5 which contained 500 micro-gram per gram of β-TCP particles. A Ti-6A1-4V dental implant with a 3.7 mm diameter and an 8 mm length was used in this study. The LIPUs had an intensity of 100mW/cm^2, a wave length of 3.0 MHz, a pulse width of 1.0 msec, a pulse cycle of 10.0 msec. Exposure of the frontal sinus was carried out bilaterally and received 2 grams of the rhGDF-5 composite. After 4, 8 and 12 weeks postsurgery, the implants were placed in the sinus graft regions. For 1 week, one sinus was stimulated by LIPUs for 10 or 20 minutes a day as the experimental group and the other sinus was not stimulated as the control group. The frontal sinuses were excised 8 weeks after dental implants placement. The bone maturation in response to LIPUs was evaluated histologically and bone contact ratio around the dental implant surface was measured. It seemed that the promoting of new bone formation was observed in the experimental groups. It was shown that the bone contact ratio of 4 weeks groups was higher the experimental groups than the control group, however there were no significant differences of bone contact ratio among three groups.

在我们之前的研究中，我们能够使用 rhGDF-5 诱导骨形成，但是该研究的结果表明，即使发生骨形成，所得的骨生长仍不成熟。本研究评估了低强度的效果。脉冲超声刺激（LIPU）可增强重组人生长分化因子 5（rhGDF-5）诱导的骨成熟。这项研究使用了体重约 12 公斤的成年比格犬。含有 rhGDF-5 的 β-TCP 基质由 rhGDF-5 制成，每克含有 500 微克 β-TCP 颗粒，其中使用直径 3.7 毫米、长度 8 毫米的 Ti-6A1-4V 牙科种植体。 LIPU 的强度为 100mW/cm^2，波长为 3.0 MHz，脉冲宽度为 1.0 毫秒，脉冲周期为10.0毫秒，暴露双侧额窦，并在术后4、8和12周后接受2克rhGDF-5复合材料，将植入物放置在窦移植区域1周。实验组每天用 LIPU 刺激 10 或 20 分钟，另一侧鼻窦不刺激作为对照组。 8 额窦被切除。牙种植体植入后几周，对 LIPU 的骨成熟反应进行了组织学评估，并测量了牙种植体表面周围的骨接触率，结果表明，在实验组中观察到了新骨形成的促进作用。 4周组骨接触率实验组高于对照组，但三组间骨接触率无显着差异。