Enhancement of Maturation Using Low-intensity Pulsed Ultrasound on Bone Formation Induced by rhGDF-5

使用低强度脉冲超声促进 rhGDF-5 诱导的骨形成的成熟

• 批准号: 18592137
• 负责人: HIROSE Yukito
• 金额: $ 2.62万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592137/
• 关键词: Regenerative Medicine; dentistry; stress; bioactivity; bone formation; bone density; LIPUs; rhGDF-5; rh-GDF-5; Regenerative Medicine; dentistry; stress; bioactivity; bone formation; bone density; LIPUs; rhGDF-5; rh-GDF-5

In our previous study, we were able to induce bone formation by using rhGDF-5. However, the results of that study showed that even though bone formation occurred, the resulting bone growth was immature. This study assessed the efficacy of the low-intensity pulsed ultrasound stimulation (LIPUs)to enhance the bone maturation that was induced by the recombinant human growth differentiation factor 5 (rhGDF-5). This study used adult beagle dogs that weighed about 12 kg. The composite of β-TCP matrix with rhGDF-5 is made of rhGDF-5 which contained 500 micro-gram per gram of β-TCP particles. A Ti-6A1-4V dental implant with a 3.7 mm diameter and an 8 mm length was used in this study. The LIPUs had an intensity of 100mW/cm^2, a wave length of 3.0 MHz, a pulse width of 1.0 msec, a pulse cycle of 10.0 msec. Exposure of the frontal sinus was carried out bilaterally and received 2 grams of the rhGDF-5 composite. After 4, 8 and 12 weeks postsurgery, the implants were placed in the sinus graft regions. For 1 week, one sinus was stimulated by LIPUs for 10 or 20 minutes a day as the experimental group and the other sinus was not stimulated as the control group. The frontal sinuses were excised 8 weeks after dental implants placement. The bone maturation in response to LIPUs was evaluated histologically and bone contact ratio around the dental implant surface was measured. It seemed that the promoting of new bone formation was observed in the experimental groups. It was shown that the bone contact ratio of 4 weeks groups was higher the experimental groups than the control group, however there were no significant differences of bone contact ratio among three groups.

在我们先前的研究中，我们能够使用RHGDF-5诱导骨形成。然而，该研究的结果表明，即使发生骨形成，骨的生长也不成熟。这项研究评估了低强度脉冲超声刺激（Lipus）的效率，以增强由重组人类生长分化因子5（RHGDF-5）诱导的骨骼成熟。这项研究使用了重约12公斤的成年小猎犬犬。具有RHGDF-5的β-TCP矩阵的复合材料由RHGDF-5制成，RHGDF-5每克每克β-TCP颗粒中包含500微克。本研究使用了直径为3.7 mm，长度为8 mm的TI-6A1-4V牙科植入物。 Lipus的强度为100MW/cm^2，波长为3.0 MHz，脉冲宽度为1.0毫秒，脉冲周期为10.0毫秒。双侧进行额窦的暴露，并获得2张RHGDF-5复合材料的图。 4、8和12周的术后，冒名顶替者被放置在鼻窦图形区域。在1周内，由于实验组和另一个窦为对照组，每天通过脂肪刺激一种鼻窦刺激10或20分钟。牙齿冒名顶替者放置8周后，正面鼻窦非常出色。通过组织学评估对脂肪的骨骼成熟，并测量牙齿植入物表面周围的骨接触比。似乎在实验组中观察到了新骨形成的促进。结果表明，4周组的骨接触率高于对照组的实验组，但是三组之间的骨接触比没有显着差异。