The analysis of the stria vascularis and the lateral wall of cochlea in the audiometric hearing loss animal model by the Arg-Vasopressin

精氨酸加压素对听力损失动物模型血管纹和耳蜗侧壁的分析

• 批准号: 18591885
• 负责人: NAGANUMA M.D. Hideaki
• 金额: $ 2.53万
• 依托单位: Kitasato University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591885/
• 关键词: Arg-vasopressin; stria vascularis; intermediate cell; edema; endolymphatic hydrops; hearing impairment; oxygen partial pressure; Meniere's disease; Arg-Vasooressin

1. Animal model study.In our previous study, it was confirmed that the hearing of the animal modes administered Arg-Vasopressin (AVP) was impaired. The lateral wall of cochlea, especially the stria vascularis in the animal model administered AVP was investigated morphologically. Wistar rats were intra-peritoneally injected AVP (0.02 units/g). The morphology in the stria vascularis in one hour after the administering AVP was investigated in electron microscopy. As the results, an intra-cellar edema was found in the intermediate cell of the stria vascularis. It is assumed that the hearing impairment induced by AVP administering is related to the morphological change in the intermediate cells. It is considered that the intra-cellular edema induce that a blood circulation in the stria vascularis is impaired, the oxygen partial pressure is decreased and the ion-metabolism is impaired. As the results, it was assumed that the hearing was impaired.2. Clinical study.We examined whether sufficie … More nt water intake is effective in the long-term control of vertigo and hearing activity in patients with Meniere's disease (MD) for whom conventional therapy has proven unsuccessful. Time-series study with historical control. Fifty-eight patients with MD in Group 1 drank 35 ml/kg/day of water for 2 years. Twenty-nine patients with MD treated with the conventional dietary and diuretic therapy for more than 2 years during 1992-99 at the same Hospital were enrolled in a historical control of Group 2. Patients in Group 1 dramatically relieved vertigo and significantly improved in the hearing of the worst pure-tone average of three frequencies (0.125, 0.25, and 0.5 kHz) (low-PTA) during the last 6 months of the study period. In contrast, patients in Group 2 became worse in both the four (0.5, 1, 2, and 4 kHz) frequency-PTA and the low-PTA, although their vertigo did improve. The number of patients whose hearing were improved, unchanged and worse were 18, 37 and 3 in Group 1 and 2, 11 and 16 in Group 2, respectively. Deliberate modulation of the intake of water may be the simplest and most cost-effective medical treatment for patients with MD.Larger studies will be needed to confirm these results in a larger patient cohort. Less

1.动物模型研究。在我们之前的研究中，证实了给予精氨酸加压素（AVP）的动物模型的听力受损。对给予AVP的动物模型的耳蜗侧壁，特别是血管纹进行了形态学研究。 Wistar大鼠腹腔注射AVP（0.02单位/g），用电子方法观察注射AVP后1小时内血管纹的形态。结果，在血管纹的中间细胞中发现了细胞内水肿，推测施用AVP引起的听力障碍与中间细胞的形态变化有关。 -细胞水肿导致血管纹的血液循环受损，氧分压降低，离子代谢受损。由此推测听力受损。2．研究。我们对梅尼埃病 (MD) 患者的眩晕和听力活动的长期控制是否有效，进行了历史对照的时间序列研究，这些患者的常规治疗已被证明不成功。第 1 组中的 8 名 MD 患者每天饮用 35 ml/kg 的水，29 名 MD 患者接受常规饮食和利尿治疗超过 2 年。 1992-99 年同一医院的患者被纳入第 2 组的历史对照。第 1 组患者的眩晕症状显着缓解，并且三个频率（0.125、0.25 和 0.5 kHz）的最差纯音平均值的听力显着改善（相比之下，在研究期间的最后 6 个月内，第 2 组的患者在四个频率（0.5、1、2 和 4 kHz）中的情况都变得更糟。频率-PTA 和低-PTA 组中，眩晕确实有所改善。第 1 组中听力改善、不变和恶化的患者人数分别为 18 例、37 例和 3 例，第 2 组中听力改善为 11 例和 16 例。对于MD患者来说，减少水的摄入量可能是最简单且最具成本效益的治疗方法。需要更大规模的研究来在更大的患者群体中证实这些结果。

项目成果

Water may cure patients with Meniere disease

• DOI: 10.1097/01.mlg.0000225904.78569.0c
• 发表时间: 2006-08-01
• 期刊: LARYNGOSCOPE
• 影响因子: 2.6
• 作者: Naganuma, Hideaki;Kawahara, Katsumasa;Okamoto, Makito
• 通讯作者: Okamoto, Makito

メニエール病、急性低音障害型感音難聴、突発性難聴の成因

梅尼埃病、急性低频感音神经性听力损失和突发性听力损失的原因

• 发表时间: 2006
• 作者: 長沼 英明;河原 克雅;徳増 厚二;橋本 晋一郎;伊藤 昭彦;落合 敦;佐藤 亮平;岡本 牧人;三枝英人;長沼英明;三枝英人;三枝英人;清野由輩;三枝英人(分担);三枝英人;長沼英明;落合敦;三枝英人;三枝英人;三枝英人;長沼英明;田沼久美子・益田律子・三枝英人;長沼英明;三枝英人;三枝英人;落合敦;長沼英明;和田昌興;伊藤昭彦;長沼英明
• 通讯作者: 長沼英明

Juvenile Meniere's disease

青少年梅尼埃病

• 发表时间: 2008
• 作者: 長沼 英明;河原 克雅;徳増 厚二;橋本 晋一郎;伊藤 昭彦;落合 敦;和田 昌興;佐藤 亮平;岡本 牧人;落合敦
• 通讯作者: 落合敦

内リンパ水腫関連疾患症例における血漿AVP値の日内変動

内淋巴积液相关疾病中血浆 AVP 水平的昼夜波动

• 发表时间: 2007
• 作者: 長沼 英明;河原 克雅;徳増 厚二;橋本 晋一郎;伊藤 昭彦;落合 敦;佐藤 亮平;岡本 牧人;三枝英人;長沼英明;三枝英人;三枝英人;清野由輩;三枝英人(分担);三枝英人;長沼英明;落合敦;三枝英人;三枝英人;三枝英人;長沼英明;田沼久美子・益田律子・三枝英人;長沼英明
• 通讯作者: 長沼英明

メニエール病(内リンパ水腫)の聴力低下メカニズムの解明をめざして

旨在阐明梅尼埃病（内淋巴积水）听力损失的机制

• 发表时间: 2007
• 作者: 長沼 英明;河原 克雅;徳増 厚二;橋本 晋一郎;伊藤 昭彦;落合 敦;佐藤 亮平;岡本 牧人;三枝英人;長沼英明
• 通讯作者: 長沼英明