Elucidation of the biosynthesis and the biological function of DKxanthenes during fruiting body formation in Myxococcus xanthus

阐明黄粘球菌子实体形成过程中 DKxanthenes 的生物合成和生物学功能

• 批准号: 53572187
• 负责人: Professor Dr. Rolf Müller
• 金额: --
• 依托单位: Helmholtz-Institut für Pharmazeutische Forschung Saarland
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/53572187?language=en
• 关键词: Elucidation; biosynthesis; biological; function; DKxanthenes

This project aims to investigate the role of a recently discovered novel class of secondary metabolites - the DKxanthenes - during fruiting body formation by the myxobacterial model organism Myxococcus xanthus. Under starvation conditions, cells enter the developmental life cycle which culminates in the differentiation of vegetative cells into stress-resistant myxospores within the fruiting body. Mutants unable to produce the DKxanthene pigments were shown to be strongly delayed in mature spore production. The underlying mechanism remains unclear, even though the lipid-like structure and the assigned antioxidative capacity of the DKxanthenes imparts a protective effect, most likely within the cell membrane. In order to understand the physiological processes underlying the absence and/or presence of the DKxanthenes, this work aims to analyze DKxanthene biosynthesis on the molecular biological level, as well as to determine the differing morphological and stress-resistance properties of M. xanthus wild type and DKxanthene-negative cells.

该项目旨在研究最近发现的一类新型次级代谢产物 - DKxanthenes - 在粘细菌模式生物 Myxococcus xanthus 子实体形成过程中的作用。在饥饿条件下，细胞进入发育生命周期，最终营养细胞分化为子实体内的抗应激粘孢子。不能产生 DKxanthene 色素的突变体在成熟孢子的产生中被证明被严重延迟。尽管 DKxanthenes 的类脂质结构和指定的抗氧化能力赋予了保护作用（很可能是在细胞膜内），但其潜在机制仍不清楚。为了了解 DKxanthene 缺失和/或存在的生理过程，本工作旨在在分子生物学水平上分析 DKxanthene 生物合成，并确定 M. xanthus 野生型的不同形态和抗逆特性。和 DKxanthene 阴性细胞。