Molecular Genetics of the Heterogeneiety of Gastrointestinal Cancer in association of cancer stem cells.

与癌症干细胞相关的胃肠癌异质性的分子遗传学。

基本信息

批准号：
18390367
负责人：
INOUE Hiroshi
金额：
$ 11.28万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390367/
关键词：
Cancer Heterogeneity DNA microarray LMD Gastric cancer Hepatocellular carcinoma Laser pressure cell transfer method Genome array CD49 抗癌剤耐性 SP

项目摘要

PurposeTo investigate the genetic base of the heterogeneity of the digestive cancer, two approaches such as 1) DNA microarray analysis combined with laser microdissection for the gene expression of the digestive tract carcinoma and 2) cancer stem cell survey have been conducted.Material and methodCell line and clinically resected tumor specimens were used for the analysis. Human 44k-DNA microarray were purchased from “Agilent technology" and microarray analysis were performed according to the company's instruction. In addition, genomic microarray were performed for 10 cases of esophageal cancer and 5 cased of heptocellular carcinoma.Results1) Early staged cancer shows no definitive different expression profile from various parts of the tumor, however, in advance staged cancer, a definitive difference of the profile was observed among the portions of the tumor, in particular, superficial luminal parts and deeper invasive portions. 2) Expression profiles differ in cases with different histological types. 3) Genomic alteration and simultaneous mRNA expression changes were observed in accordance with the histological development of the tumor. In heptocellular carcinoma, peripheral portion of the tumor shows very faint alteration in both mRNA and genomics. However, in the central portion that reciprocally later developed, histologically showed more aggressive pattern and also manifested very variable alterations in both mRNA and genomics.DiscussionDNA microarray analysis combined with laser microdissection for the gene expression as well as genomic alteration had very powerful potential for the analysis of histologically complexed tumor. More detailed study will clarify the perplexed nature of the human carcinogenesis.

目的者研究了消化癌异质性的遗传基础，两种方法，例如1）DNA微阵列分析与激光显微解剖相结合，用于消化道癌的基因表达和2）癌症干细胞调查已进行。根据公司的指示进行了“安捷伦技术”和微阵列分析。此外，对10例食管癌和5例七个甲状腺癌癌进行了基因组微阵列1）早期阶段癌症显示，与肿瘤的各个部位进行了明确的表达概况，但是，事先观察到肿瘤的特征的明确差异在肿瘤的各个部分中均存在明确的差异。 2）在不同组织学类型的情况下，表达曲线不同。 3）根据肿瘤的组织学发展观察到基因组改变和简单的mRNA表达变化。在七个细胞性癌中，肿瘤的周围部分在mRNA和基因组学中均显示出非常微弱的改变。然而，在相互反复发展的中心部分中，组织学表现出更具侵略性的模式，并且在mRNA和基因组学上都表现出非常可变化的变化。DiscussionDNA微阵列分析与激光显微解剖相结合基因表达以及基因组变量以及基因组改变具有非常强大的潜在潜力，可用于分析组织学复杂的肿瘤。更详细的研究将阐明人类致癌的困惑性质。