Study on the Dynamic Properties of Living-Cells from the viewpoint of Intelligent Vibration-Reducible Structure

从智能减振结构角度研究活细胞的动态特性

基本信息

批准号：
18360111
负责人：
MORISHITA Shin
金额：
$ 10.67万
依托单位：
Yokohama National University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Living creatures are fundamentally composed of cells, which may adapt to their environment; they get together finding the same kind of cells, exclude inconvenient stimulus to them, and keep the best condition for life conservation. Though the cells have been treated in biology, medical science, or bio-chemistry, they may be paid attention by mechanical engineers when they are regarded as a prototype of micro-machine. But, almost nothing has been studied as for the mechanical properties of cells. This project is concentrated to reveal mechanical properties of living cells and they are applied to construct more intelligent structures. As living cells, a bone related cell are used in this study.Bone cells have adaptability to the surrounding mechanical condition. Osteoblasts, which are responsible for bone formation, were reported that they responded to mechanical vibration and changed the cell proliferation and the amount of generated bone mass. In order to find the effective stimulation … More on osteogenesis, it is necessary to understand the mechanical properties such as elasticity or viscosity of the cells in the view of mechanical engineering. In the case of bone cells, it is important to understand the mechanical properties under adhesive condition because they attach on surfaces of bone matrices. In this study, I measured dynamic viscoelasticity of a cultured osteoblast, MC3T3-E1, under adhesive condition by applying sinusoidal deformation. Using these experimental results, we derived Voigt model for viscoelasticity and identified the value of each parameter.Next, the effect of mechanical stimulation was evaluated. Mechanical stimulation to bones affects osteogenesis such as decrease of bone mass of astronauts under zero gravity and fracture repair with ultrasound devices. Bone cells have been reported to sense and response to mechanical stimulation at cellular level morphologically and metabolically. In the view of mechanical vibrations, bone cells are deformed according to mechanical stimulation and their mechanical characteristics. It was reported that viscoelasticity of cells was measured using tensile and creep tests and that there was likely natural frequency and nonlinearity of cells in the sense of structural dynamics. It suggests that there is effective frequency and amplitude of mechanical stimulation on osteogenesis by bone cells. In this study, sinusoidal inertia force was applied to cultured osteoblasts, MC3T3-E1, and effects of frequency and acceleration amplitude of mechanical vibration on the cells were investigated in respect of the cell proliferation, bone matrix generation and alkaline phosphatase (ALP) gene expression. Less

生物体本质上是由细胞组成的，它们可以通过生物学、医学的处理，找到同类的细胞，排除不便的刺激，保持生命的最佳状态。无论是科学，还是生物化学，当它们被视为微型机器的原型时，它们可能会受到机械工程师的关注，但是，对于细胞的机械性能几乎没有任何研究，这个项目集中于揭示机械性能。活细胞，它们被用来构建更智能的作为活细胞，本研究中使用了骨相关细胞。据报道，负责骨形成的成骨细胞对周围的机械条件具有适应性，它们对机械振动做出反应并改变了细胞增殖和骨形成。为了找到对成骨的有效刺激，有必要从机械工程的角度了解细胞的弹性或粘度等机械特性。了解机械性能很重要粘合条件，因为它们附着在骨基质的表面上。在这项研究中，我通过应用正弦变形测量了培养的成骨细胞 MC3T3-E1 在粘合条件下的动态粘弹性。接下来，评估机械刺激对骨骼的影响，例如零重力下宇航员骨量的减少和骨折修复。据报道，骨细胞在形态和代谢方面对机械刺激做出反应。据报道，骨细胞根据机械刺激及其机械特性而变形。使用拉伸和蠕变测试进行测量，并且在结构动力学意义上存在细胞的固有频率和非线性，这表明在本研究中，骨细胞的成骨存在有效的机械刺激频率和幅度。对培养的成骨细胞MC3T3-E1施加惯性力，考察机械振动的频率和加速度幅度对细胞增殖、骨基质生成和碱性磷酸酶（ALP）基因表达的影响。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mechanical Properties of a Cultured Osteoblast

培养的成骨细胞的机械特性

DOI：
发表时间：
2005
期刊：
Proceedings of International Joint Conference of JFSIMS & SMEBA 2005 (CD-ROM)
影响因子：
0
作者：
Takafumi Onishi;Toshihiko Shiraishi;Shin Morishita
通讯作者：
Shin Morishita

Visco-elastic Properties of Cultured Osteoblast under Adhesive Condition

粘附条件下培养成骨细胞的粘弹性特性

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Takafumi;Ohnishi
通讯作者：
Ohnishi

Effects of Amplitude and Frequency of Vibration Stimulation on Cultured Osteoblasts

振动刺激幅度和频率对培养成骨细胞的影响

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Wei LIU;Kazuyuki TAKASE;Tetsuo Shikata;高木裕太;Yuta Takagi;Kei Suzuki;白石俊彦;鈴木圭;高木裕太;白石俊彦;鹿田哲生;鹿田哲生;松岡太一;Tetsuo Shikata
通讯作者：
Tetsuo Shikata

接着状態における培養骨芽細胞の粘弾性の測定

贴壁状态培养成骨细胞粘弹性的测量