BIOLOGICAL MONITORING OF EXPOSURE TOomicron-DICHLOROBENZENE

太微米二氯苯暴露的生物监测

• 批准号: 07457115
• 负责人: KUMAGAI Shinji
• 金额: $ 1.6万
• 依托单位: OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH,SCIENTIFIC RESEARCH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457115/
• 关键词: omicron-DICHLOROBEZENE; URINARY METABOLITE; DICHLOROCATECHOL; DICHLOROPHENOL; BIOLOGICAL MONITORING

1) Identification of urinary metabolites of human subjects exposed to omicron-dichlobenzene (omicron-DCB)Urine samples were collected from three male workers exposed to omicron-DCB and they were analyzed using a gas chromatograph-mass spectrometer. 2,3-dichlorophenol (2,3-DCP), 3,4-dichlorophenol (3,4-DCP), 3,4-dichlorocatechol (3,4-DCC) and 4,5-dichlorocatechol (4,5-DCC) were detected in the all samples.2) Quantitative determination of urinary metabolites of omicron-DCB using a high performance liquid chromatograph (HPLC)First, urinary conjugated metabolites were hydrolyzed by concentrated hydrochloric acid, and the metabolites were extracted with diethylether. Next, 0.5 ml of the ethylether layr was separated, and 2.0 ml of methyl alcohol was added. The HPLC was connected to a column packed with LiChrospher 100 RP-18. For DCCs, the mobile phase was a mixture of acetonitrile, acetic acid and water (1 : 12 : 87). For DCPs, the mobile phase was a mixture of methyl alcohol, acetic acid a … More nd water (33 : 5 : 62). The effuents were monitored at 285 nm. The calibration curves were linear from 10 to 100 mg/l. The mean recovery rates of DCCs and DCPs were more than 90%.3) Relation between exposure to omicron-DCB and urinary metabolitesIn a plant symthesizing intermediate products for dyes, the 8-hr time weighted average (8-hr TWA) value for the exposure concentration of omicron-DCB and concetrations of urinary 3,4-DCC,4,5-DCC,2,3-DCP and 3,4-DCP were determined for 28 male workers. The concetrations of 3,4-DCC,4,5-DCC,2,3-DCP and 3,4-DCP inurine collected at the end of the workshift were shown to be linearly correlated to the 8-hr TWA value for the exposure concentration of omicron-DCB.Concequently, biological exposure monitoring can be done.4) Quantitative Determination of Urinary Metabolites of omicron-DCB Using a Gas Chromatograph (GC)Urine treatment was the same as that in the above HPLC method. The GC was connected to a capillary column was Shimadzu CBP-20. Helium was used as the carrier gas. The column temperature was programd from 50 to 220ﾟC at 3ﾟC/min. The injector and detector temperatures were 250ﾟC.The calibration curves were linear from 10 to 100mg/l. The mean recovery rates of 2,3-and 3,4-DCPs were more than 90%. Less

1) 暴露于 omicron-二氯苯 (omicron-DCB) 的人类受试者尿液代谢物的鉴定 从暴露于 omicron-DCB 的三名男性工人中采集尿液样本，并使用气相色谱-质谱仪进行分析 2,3-二氯苯酚 (omicron-DCB)。 2,3-DCP)、3,4-二氯苯酚(3,4-DCP)、3,4-二氯儿茶酚所有样品中均检测出 omicron-DCB (3,4-DCC) 和 4,5-二氯儿茶酚 (4,5-DCC)。 2) 使用高效液相色谱仪 (HPLC) 定量测定 omicron-DCB 尿代谢物用浓盐酸水解结合的代谢物，然后用二乙醚提取代谢物0.5ml。分离乙醚层，加入2.0ml甲醇。对于DCC，流动相是乙腈、乙酸和水(1:12:)的混合物。 87) 对于 DCP，流动相是甲醇、乙酸和水的混合物 (33 : 5 : 62) 在 285 nm 处监测流出物，DCC 和 DCP 的平均回收率超过 90%。3) omicron-DCB 暴露与尿代谢物之间的关系。合成染料中间产品的工厂，暴露浓度的 8 小时时间加权平均值 (8 小时 TWA) 值测定了 28 名男性工人的 omicron-DCB 以及尿中 3,4-DCC、4,5-DCC、2,3-DCP 和 3,4-DCP 的浓度。 3,4-DCC、4,5- 的浓度。轮班结束时收集的 DCC、2,3-DCP 和 3,4-DCP 尿液与 8 小时 TWA 值呈线性相关4)采用气相色谱仪(GC)定量测定omicron-DCB的尿液代谢物。尿液处理与上述HPLC方法相同。连接到毛细管柱的是 Shimadzu CBP-20，用作载气。柱温设定为 50 到 100。 220°C，3°C/min。进样器和检测器温度为 250°C。校准曲线从 10 到 100mg/l 呈线性。2,3-和 3,4-DCP 的平均回收率超过。 90%.以下

项目成果

Shinji Kumagai, Ichiro Matsunaga: "Identification of urinary metabolites of human subjects exposed to omicron-dichlorobenzene" Int Arch Occup Environ Health. 67. 207-209 (1995)

Shinji Kumagai、Ichiro Matsunaga：“暴露于 omicron-二氯苯的人体尿液代谢物的鉴定”Int Arch Occup Environ Health。

Shinji Kumagai: "Relations between exposure to o-dichlorobenzene and concentrations of urinary metabolites." J Occup Health. 39. 124-129 (1997)

Shinji Kumagai：“接触邻二氯苯与尿液代谢物浓度之间的关系。”