Study on early diagnostic system for cervical cancer with using liquid-based cytology samples

液基细胞学样本宫颈癌早期诊断系统的研究

基本信息

批准号：
18590319
负责人：
SANO Takaaki
金额：
$ 2万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590319/
关键词：
HPV Real-time PCR copy number p16 L1 capsid protein virus status cervical cancer dysplasia 液状化細胞診検体免疫染色病理診断 CIN 細胞診断学リアルタイムPCR ウイルス量細胞診液状化検体

项目摘要

1. Human papillomavirus (HPV) 16 DNA can be integrated into the DNA of cells, thereby disrupting E2 geneexpression, which leads to increased expression of the E6 and E7 viral oncogenes and progression to cancer. Liquid-based cytology samples were examined by quantitativereal-time polymerase chain reaction. The HPV 16 viral load per cell decreased from a low-grade squamous intraepithelial lesion (LSIL) to a cancerous lesion. The average HPV 16 DNA copy numbers for three different HPV 16 integration statuses were 64.1 for the episomal form, 465.5 for the mixed form, and 0.4 for the integrated form. Furthermore, the mean age of patients with the pure integrated form of HPV 16 was more than 10 years older than those of patients with the episomal and mixed forms. Quantitative real-timePCR appears to be a useful method for quantitative and physical status analyses of HPV in cervical cancer screening with LBC samples.2. HPV L1 capsid protein is expressed together with the productionof infectious viral particles, but its expression and relation to p16 expression, which has been a surrogate marker for HPV infection in cervix. Immunochemical analyses using antibodies against L1 capsid protein and p16 protein were carried out on LBC materials. L1 capsid protein was positive in 30% of LSILs and 12% of HSILs, butin 0% of SCCs. In contrast, p16 protein was positive in 55% of LSILs, 100% of HSILs, and 100% of SCCs. Expression of L1 capsid protein decreased with lesion progression from LSILs to HSILs and SCCs, whereas p16 protein was positive in all HSILs and SCCs. The correlation between Ll and p16 expressions suggests that L1(-)/p16(+) cases have the potential for progression, whereas L1(+)/p16(-) andL1 (-)/p16(-) cases may be nonprogressive lesions or potentially in remission.

1。人乳头瘤病毒（HPV）16 DNA可以整合到细胞的DNA中，从而破坏E2 Geneexpression，从而导致E6和E7病毒性肿瘤基因的表达增加，并发展为癌症。通过定量时间聚合酶链反应检查基于液体的细胞学样品。每个细胞的HPV 16病毒负荷从低级鳞状上皮内病变（LSIL）降低到癌变。三种不同的HPV 16积分状态的平均HPV 16 DNA拷贝数为64.1，混合形式为465.5，集成形式为0.4。此外，HPV 16的纯整合形式患者的平均年龄比具有偶发和混合形式的患者大10岁以上。定量实时PPCR似乎是用LBC样品筛查HPV定量和物理状态分析的有用方法。2。 HPV L1衣壳蛋白与感染性病毒颗粒的产生一起表达，但其表达和与p16表达的关系，它一直是子宫颈HPV感染的替代标记。使用针对L1衣壳蛋白和p16蛋白的抗体进行免疫化学分析，以LBC材料进行。在30％的LSIL和12％的HSIL中，L1衣壳蛋白为阳性，butin占SCC的0％。相比之下，55％的LSIL，100％的HSIL和100％的SCC中的P16蛋白为阳性。 L1衣壳蛋白的表达随着从LSIL到HSIL和SCC的病变的进展而降低，而P16蛋白在所有HSIL和SCC中均为阳性。 LL和p16表达式之间的相关性表明L1（ - ）/p16（+）情况具有进展的潜力，而L1（+）/p16/p16（ - ）和L1（ - ）/p16（ - ）/p16（ - ）病例可能是非预性病变或有可能缓解。