Role of gonadal steroids on the in vivo acetylcholine release in the hippocampus

性腺类固醇对海马体内乙酰胆碱释放的作用

基本信息

批准号：
18590219
负责人：
MITSUSHIMA Dai
金额：
$ 2.5万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590219/
关键词：
aPKC PAR-1 epithelial cells polarity basal laminina dvstroglycan cancer muscular dystrophy 海馬行動リズム

项目摘要

Septo-hippocampal cholinergic neurons play a significant role in the spatial learning and memory function. We recently revealed a marked sex difference in the profile of 24-h acetylcholine (Ach) release in the hippocampus. In experiment I, to examine the role of sex hormones in the 24-h ACh release in the hippocampus, in vivo microdialysis study was performed in intact, gonadectomized (Gdx), gonadectomized testosterone-primed (Gdx+T), and gonadectomized estadiol-17β-primed (Gdx+E) rats, under the freely moving condition for more than 24 hours. The dialysate was collected from the dorsal hippocampus at 20-min intervals, and spontaneous locomotor activity was simultaneously monitored in the same subject. Although the sex-specific ACh release profile was observed in intact male and cycling female rats, the ACh release during the dark phase was severely attenuated in both sexes of Gdx rats. Both Gdx+T male rats and Gdx+E female rats successfully restored the ACh release during the dark pha … More se, while neither Gdx+T female rats nor Gdx+E male rats restored each profile. Although Gdx significantly decreased in the correlation coefficient between the ACh release and the spontaneous locomotor activity, both Gdx+T male rats and Gdx+E female rats successfully restored the correlation coefficient. In experiment II, to further examine the short-term effect of sex hormones on the ACh release in the hippocampus, testosterone (2 mg) or estadiol-17β (40 μg) was subcutaneously injected in Gdx male and Gdx female rats, respectively. Testosterone injection in Gdx male rats gradually, but significantly increased the ACh release within the first few hours, and it restored the ACh release up to the level in intact male rats within 10 hours. Similarly, estradiol-17β injection in Gdx female rats also increased the ACh release within the first few hours, and restored the ACh release up to the level in cycling female rats within 10 hours. Oil injection showed no significant effects on the ACh release. Present results demonstrated that (1) testosterone in male rats as well as estadiol-17β in female rats are essential to maintain the normal profile of ACh release in the hippocampus, (2) sex hormones may have a short-term effect on the ACh release. Less

SEPTO-HAPPOCAMPAL胆碱能神经元在空间学习和记忆功能中起着重要作用。我们最近揭示了海马中24小时乙酰胆碱（ACH）释放的性别差异。在实验I中，为了检查性恐怖在海马24-H ACH释放中的作用，在完整的，性腺切除术（GDX），Gonadectomized睾丸激素（GDX+T）（GDX+T）和Gonadectomiziol-17β提示的情况下，进行了24小时的频率（GDX+T），对24小时的限制进行了24小时，进行了体内微透析研究。透析液以20分钟的间隔从背侧海马收集，并在同一受试者中简单地监测赞助运动活性。尽管在完整的雄性和骑自行车的雌性大鼠中观察到了性别特异性的ACH释放曲线，但在GDX大鼠的两性性别中，黑暗阶段的ACH释放都严重减弱。 GDX+T雄性大鼠和GDX+E雌性大鼠在黑暗的PHA中成功恢复了ACH释放……更多，而GDX+T雌性大鼠也没有GDX+E雄性大鼠恢复每个轮廓。尽管GDX在ACH释放与发起人的自发运动活性之间的相关系数中显着降低，但GDX+T雄性大鼠和GDX+E雌性大鼠均成功恢复了相关系数。在实验II中，分别在GDX雄性和GDX雌性大鼠中皮下注射了性恐怖对性恐怖对ACH释放的短期影响。 GDX雄性大鼠的睾丸激素注射逐渐逐渐增加，但在最初的几个小时内显着增加了ACH的释放，并且将ACH释放恢复到了10小时内完整雄性大鼠的水平。同样，GDX雌性大鼠中的雌二醇-17β注射也增加了ACH在最初的几个小时内释放，并将ACH的释放恢复到10小时内循环雌性大鼠的水平。目前的结果表明，（1）雄性大鼠的睾丸激素以及雌性大鼠的estadiol-17β对于维持海马中ACh释放的正常释放至关重要，（2）性恐怖可能对ACH释放产生短期影响。较少的