Study for detecting quantitative trait loci affecting eggshell strength

影响蛋壳强度的数量性状位点检测研究

• 批准号: 18580285
• 负责人: TAKAHASHI Hideaki
• 金额: $ 2.34万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580285/
• 关键词: chicken; egg; eggshell; QTL; SNP; protein mutation

Improvement of commercial layers is approaching breeding goals in the viewpoint of productivity. Next targets for breeding are reducing economic loss. Broken and cracked eggshells cause major economic loss for egg production. Roland (1988) estimated that the rate of inadequate shells was 7.7% at all production stages, and then 5.16% of broken eggs in the commercial activities, both caused totally 13-20% of egg loss. Therefore, breeding for egg shell strength is needed. The aim of this study is to identify quantitative trait loth (QTL) affecting egg shell strength in White Leghorn.An F2 population of 262 hens, obtained by crossing a strong egg shell line with a weak egg shell line of White Leghorn, was used for detecting quantitative trait loci affecting egg character. The two lines were developed from the same line, D2(Okazaki Station, National Livestock Breeding Center of Japan), by two-way selection for egg shell strength with nondestructive deformation Nirasawa et al., 1998). 1,014 microsatellite markers including 696 ABR markers were used, which are covered whole chicken genome. QTL analysis was performed using QTL Express (Seaton et al,1994)Of the markers u sed, 35 markers were informative and mapped on 10 autosomal linkage groups. There was no informative marker on chromosome Z. The QTL for 7 traits, i. e., body weight (BW), egg weight (EVE, short length of egg (SL), long length of egg (LT..), shell strength (SS), shell thickness (ST) and shell weight (SW)) were identified. Highly significant (p<0.01) QTL for ST, SW and LL were identified a region flanking with ABR0362 and ABR0545 on chromosome 9. These QTL are good candidates for the reduction of broken and cracked eggs in wmmercial layers by marker assisted selection (MAS).

从生产力的角度来看，商品蛋鸡的改进正在接近育种目标。育种的下一个目标是减少经济损失。破碎和破裂的蛋壳给鸡蛋生产造成重大经济损失。 Roland（1988）估计，在所有生产阶段，蛋壳不合格率为7.7%，商业活动中的破蛋率为5.16%，两者合计造成鸡蛋损失13-20%。因此，需要进行蛋壳强度育种。本研究的目的是鉴定影响白来航蛋壳强度的数量性状 (QTL)。通过将白来航强蛋壳品系与弱蛋壳品系杂交获得的 F2 群体 262 只母鸡，用于检测影响蛋性状的数量性状位点。这两个品系是从同一个品系 D2（日本国家畜牧中心冈崎站）通过无损变形蛋壳强度双向选择发展而来的（Nirasawa 等，1998）。使用1,014个微卫星标记，其中包括696个ABR标记，覆盖鸡全基因组。使用QTL Express（Seaton等人，1994）进行QTL分析。在使用的标记中，35个标记具有信息性并且定位在10个常染色体连锁群上。 Z染色体上没有信息标记。7个性状的QTL，即。即体重（BW）、蛋重（EVE、蛋短长（SL）、蛋长长（LT..）、蛋壳强度（SS）、蛋壳厚度（ST）和蛋壳重量（SW））被识别出来。在第 9 号染色体上 ABR0362 和 ABR0545 侧翼的区域中鉴定出了 ST、SW 和 LL 的高度显着性 (p<0.01) QTL。这些 QTL 是通过标记辅助选择 (MAS) 减少商业蛋鸡破损蛋的良好候选者。 。

项目成果

Identification of quantitative trait loci affecting shank length, body weight and carcass weight from the Japanese cockfighting chicken breed, Oh-Shamo (Japanese Large Game)

• DOI: 10.1159/000103190
• 发表时间: 2007-07
• 期刊: Cytogenetic and Genome Research
• 影响因子: 1.7
• 作者: M. Tsudzuki;S. Onitsuka;R. Akiyama;M. Iwamizu;N. Goto;M. Nishibori;H. Takahashi;A. Ishikawa

鶏卵の卵殻強度に関わる遺伝子変異の検出方法

检测鸡蛋中与蛋壳强度相关的基因突变的方法

• 发表时间: 2008