Study for detecting quantitative trait loci affecting eggshell strength

影响蛋壳强度的数量性状位点检测研究

• 批准号: 18580285
• 负责人: TAKAHASHI Hideaki
• 金额: $ 2.34万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580285/
• 关键词: chicken; egg; eggshell; QTL; SNP; protein mutation

Improvement of commercial layers is approaching breeding goals in the viewpoint of productivity. Next targets for breeding are reducing economic loss. Broken and cracked eggshells cause major economic loss for egg production. Roland (1988) estimated that the rate of inadequate shells was 7.7% at all production stages, and then 5.16% of broken eggs in the commercial activities, both caused totally 13-20% of egg loss. Therefore, breeding for egg shell strength is needed. The aim of this study is to identify quantitative trait loth (QTL) affecting egg shell strength in White Leghorn.An F2 population of 262 hens, obtained by crossing a strong egg shell line with a weak egg shell line of White Leghorn, was used for detecting quantitative trait loci affecting egg character. The two lines were developed from the same line, D2(Okazaki Station, National Livestock Breeding Center of Japan), by two-way selection for egg shell strength with nondestructive deformation Nirasawa et al., 1998). 1,014 microsatellite markers including 696 ABR markers were used, which are covered whole chicken genome. QTL analysis was performed using QTL Express (Seaton et al,1994)Of the markers u sed, 35 markers were informative and mapped on 10 autosomal linkage groups. There was no informative marker on chromosome Z. The QTL for 7 traits, i. e., body weight (BW), egg weight (EVE, short length of egg (SL), long length of egg (LT..), shell strength (SS), shell thickness (ST) and shell weight (SW)) were identified. Highly significant (p<0.01) QTL for ST, SW and LL were identified a region flanking with ABR0362 and ABR0545 on chromosome 9. These QTL are good candidates for the reduction of broken and cracked eggs in wmmercial layers by marker assisted selection (MAS).

在生产力的角度，商业层的改善正在实现繁殖目标。繁殖的下一个目标是减少经济损失。破裂和破裂的蛋壳会导致卵产量的重大经济损失。罗兰（Roland，1988）估计，在所有生产阶段，壳的速率不足均为7.7％，然后在商业活动中占5.16％的卵子，两者完全造成卵损失的13-20％。因此，需要为蛋壳强度繁殖。这项研究的目的是鉴定影响白色leghorn的蛋壳强度的定量性状loth（QTL）。通过越过坚固的蛋壳线和弱蛋壳的白色leghorn的蛋壳线，获得了262个母鸡的F2种群，用于检测定量性状特征，影响卵特征。这两条线是从同一条线D2（日本国家牲畜育种中心的Okazaki站（Okazaki Station）开发的，它通过双向选择蛋壳强度，没有损坏变形Nirasawa等，1998）。使用了包括696个ABR标记的1,014个微卫星标记，这些标记涵盖了整个鸡基因组。使用标记物的QTL Express（Seaton等，1994）进行了QTL分析，有35个标记信息丰富，并在10个常染色体链接组上映射。染色体Z上没有信息的标记。QTL的7个特征，即。例如，确定了体重（BW），鸡蛋重量（EVE，短长度（SL），鸡蛋长度（Lt ..），壳强度（SS），壳厚度（ST）和壳重量（SW））。 ST，SW和LL的高度显着（p <0.01）QTL被鉴定出染色体9上的ABR0362和ABR0545的区域。这些QTL是通过标记辅助选择（MAS）减少WMM商业层中损坏和破裂的鸡蛋的良好候选者。

项目成果

Identification of quantitative trait loci affecting shank length, body weight and carcass weight from the Japanese cockfighting chicken breed, Oh-Shamo (Japanese Large Game)

• DOI: 10.1159/000103190
• 发表时间: 2007-07
• 期刊: Cytogenetic and Genome Research
• 影响因子: 1.7
• 作者: M. Tsudzuki;S. Onitsuka;R. Akiyama;M. Iwamizu;N. Goto;M. Nishibori;H. Takahashi;A. Ishikawa

鶏卵の卵殻強度に関わる遺伝子変異の検出方法

检测鸡蛋中与蛋壳强度相关的基因突变的方法

• 发表时间: 2008