生物時計と潮汐によるサンゴ礁棲ベラ類の産卵時刻あわせ

利用生物钟和潮汐协调珊瑚礁濑鱼物种的产卵时间

• 批准号: 18580189
• 负责人: TAKEMURA Akihiro
• 金额: $ 2.51万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580189/
• 关键词: Coral reef; Tidal rhythm; Biological clock; Wrasse; Spawning; Histology; ステロイドホルモン

Many wrasses in coral reefs exhibit daily spawning that peaks around daytime high tide. In this study, we examined tidal-related ovarian development in the threespot wrasse Halichoeres trimaculatus, which is a common species in coral reefs of the Indo-Pacific Ocean. When the fish were collected in the morning at different tidal phases, the gonadosomatic index (GSI) and ovarian histology changed; concomitant with increases in GSI towards high tide, a clutch of the most advanced oocytes developed from vitellogenic to maturation stages. Ovulated eggs and post-ovulated follicles (POF) existed in most ovaries around high tide, but only POF remained around ebb tide, suggesting that spawning occurred during or after high tide. We noticed that tidal-related spawning was remarkable in the morning and that most ovaries collected at afternoon high tide exhibited post-spawning features. It was suggested that certain labrid species possess plasticity with regard to their spawning time and utilize potent environmental cues for their reproductive success. When the ovarian pieces were incubated with precursor steroids, high conversion of testosterone to 17β-estradiol occurred during high and ebb tides, while that of 17α-hydroxyprogesterone to 17α,20β-dihydroxy-4-pregnen-3-one and 17α,20β,21-trihydroxy-4-pregnen-3-one was observed during low and flood tides. Incubation of the ovarian pieces with human chorionic gonadotropin resulted in similar fluctuations of the steroid hormones with tide. Production of these steroid hormones correlated with oocyte development in the ovaries and was probably regulated by gonadotropin. These results demonstrate that the daily cycle is fundamental for oocyte development, and the tidal cycle is superimposed on this process.

珊瑚礁中的许多包裹都暴露了每日产卵，这些产卵在白天高潮时达到顶峰。在这项研究中，我们检查了三epot膜中与潮汐相关的卵巢发育包裹halichoeres trimaculatus，这是印度太平洋海洋珊瑚礁中的一种常见物种。当早晨在不同的潮汐阶段收集鱼时，促性腺瘤指数（GSI）和卵巢组织学发生了变化。随着GSI向高潮的增加，最先进的卵母细胞从植物到成熟阶段发展起来。大多数卵巢中都存在排卵的卵和卵形后的卵泡（POF），但在高潮周围，只有pof仍留在潮汐周围，这表明在高潮期间或之后发生了产卵。我们注意到，早晨，与潮汐有关的产卵非常出色，大多数卵巢在下午的高潮后产卵后特征收集。有人提出，某些唇样在产卵的时间方面具有可塑性，并利用有效的环境线索来取得生殖成功。当将卵巢碎片与前体立体定向一起孵育时，在高潮和潮汐潮时，睾丸激素高转化为17β-雌二醇，而在低潮中观察到了17α-羟基孕酮，而在17α-羟基孕酮中，在低潮和洪水中观察到了20β-二羟基-4-pregnen-3-pregnen-3-pregnen-3-碳。将卵巢碎片与人绒毛膜促性腺激素孵育，从而导致类似的类固醇马与潮汐发生相似的波动。这些类固醇马的产生与卵巢中的卵母细胞发育相关，可能受到促性腺激素的调节。这些结果表明，每日周期对于卵母细胞发育至关重要，并且在此过程中叠加了潮汐周期。

项目成果

サンゴ礁に潜む月世界

隐藏在珊瑚礁中的月球世界

• 发表时间: 2008
• 作者: Akihiro Takemura;Rena Oya;Yoriko Shibata;Yoko Enomoto;Miyuki Uchimura;Shigeo Nakamura;竹村明洋
• 通讯作者: 竹村明洋

熱帯性ベラ類の潜砂行動と潮汐性産卵

热带濑鱼物种的沙潜行为和潮汐产卵

• 发表时间: 2007
• 作者: 竹村;他
• 通讯作者: 他

Role of tidal cycle in gonadal development and spawning of the tropical Wrasse Halicoeres trimaculatus

潮汐周期在热带濑鱼性腺发育和产卵中的作用

• 期刊: Zoological Science (in press)
• 作者: AKihiro Takemura;Rena Oya;Yoriko Shibata;Yoko Enomoto;Miyuki Uchimura;Shigeo Nakamura
• 通讯作者: Shigeo Nakamura

Role of tidal cycle in gonadal development and spawning of the tropical wrasse Halichoeres trimaculatus

潮汐周期在热带濑鱼 Halichoeres trimaculatus 性腺发育和产卵中的作用

• 期刊: Zoological Science (印刷中)
• 作者: Takemura;et al.
• 通讯作者: et al.

Diurnal and circadian regulation of a melatonin receptor, MT1, in the golden rabbitfish, Siganus guttatus.

金兔鱼 Siganus guttatus 褪黑素受体 MT1 的昼夜节律调节。

• 发表时间: 2007
• 期刊: General and Comparative Endocrinology 150
• 作者: Park;et. al.
• 通讯作者: et. al.