Development of temperature and pressure variable aqueous biphasic extraction system with superheated water

过热水温压可变水相双相萃取系统的开发

• 批准号: 18550081
• 负责人: SHIBUKAWA Masami
• 金额: $ 2.57万
• 依托单位: Saitama University (2007)Nihon University (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18550081/
• 关键词: aqueous biphasic extraction; polyethylene glycol; superheated water; hydrophobic hydration; separation selectivity; aqueous biphasic extraction; polyethylene glycol; superheated water; hydrophobic hydration; separation selectivity

Aqueous two-phase systems (ATPS) are formed by mixing water, certain inorganic salts and water-soluble polymers and can be useful for the separation of various compounds. Polyethylene glycol (PEG) is nontoxic, nonflammable and nonvolatile so that the PEG-based ATPS can be regarded as environmentally friendly.We have developed an ATPS extraction device which enables one to carry out extraction at elevated temperature in order to enhance the separation selectivity and expand the applicability of ATPS. The distribution behavior of n-alcohols, ketones and nitroalkanes in ATPS formed from PEG and Na_2SO_4 was investigated from the viewpoints of interaction of the solutes with the polymer solution phase and the salting-out effect. The results obtained revealed that the separation selectivities exerted by the PEG-based ATPS and the PEG gel system are approximately the same. Differential scanning calorimetry studies on aqueous PEG solutions suggested that PEG polymer forms a hydration structure of which the composition is 50 % (w/w) PEG or the hydration number per ethylene oxide is 2.4 and the separation selectivity of the PEG-water systems can be ascribed to partition of solute compounds into the hydrated PEG polymer structure. Furthermore, it has been demonstrated that the separation selectivity of the ATPS depends strongly on temperature.Furthermore, in order to clarify the contribution of hydrophobic moieties of polymers on the separation selectivity of ATPS, the retention behavior of inorganic anions in reversed-phase liquid chromatography was investigated using the columns packed with alkyl bonded silica particles. From the results obtained, it has been concluded that the solvated water phase is formed on the surface of alkyl-bonded silica and exhibits an affinity to solute compounds different from that of the bulk liquid water.

通过混合水，某些无机盐和水溶性聚合物来形成两相系统（ATP），可用于分离各种化合物。聚乙烯乙二醇（PEG）是无毒的，不可氟的和非挥发性的，因此可以将基于PEG的ATP视为环保的ATP。我们已经开发了一种ATPS提取装置，使一个人能够在升高的温度下进行提取，以提高分离性的选择性并扩展了ATP的适用性。从溶质与聚合物溶液相的相互作用的角度研究了由PEG和Na_2SO_4形成的ATP中N-醇，酮和硝基烷的分布行为。获得的结果表明，基于PEG的ATP和PEG凝胶系统施加的分离选择性大致相同。对水钉溶液的差异扫描量热法研究表明，钉聚合物形成一种水合结构，其组成为50％（w/w）PEG或每个氧化乙烷的水合数为2.4，并且可以将PEG - 含量系统的分离选择性归因于溶质化合物的水合PEG POLYMERTUCTION。此外，已经证明，ATP的分离选择性在很大程度上取决于温度。为了澄清疏水聚合物对ATP的分离选择性的贡献，使用与柱状型的柱状色谱物相包含Alky-procked contical inica inica inica inica inica inica incothice contected complase液体液体中的无机含量的保留行为。从获得的结果中可以得出结论，溶剂化的水相在烷基键的二氧化硅表面形成，并具有与溶质化合物的亲和力不同，与大量液态水不同。