STUDY ON ANTISEPTIC EFFECT OF SILVER AND COPPER IONS AGAINST LEGIONELLA IN HOT SPRING WATERS

银、铜离子对温泉水中军团菌抗菌作用的研究

基本信息

批准号：
18510035
负责人：
KATO Naoyuki
金额：
$ 2.64万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18510035/
关键词：
LEGIONELLA HOT SPRINNG SILVER ION COPPER ION AMOEBA TROPHOZOITE CYST ANTISEPTIC EFFECT 殺菌作用循環装置温泉環境学

项目摘要

1. We studied the influence of chemical composition of hot springs to the antiseptic effect of silver ion.(1) The antiseptic effect of silver ion delayed according to-increases of chloriide and sulfuric acid ions in hot spring. (2) Some hot spring did not show any antiseptic effect despite enough silver ion concentration. The cause will be attributed to that silver ion formed complex with some kind of hot spring components.2. Antiseptic effect of silver ion against legionella cells in amoeba or amoeba alone was studied.(1) While legionella cells in amoeba were not killed at the concentration of 0.05 ppm of silver ion, silver ion of 2.5ppm showed an antiseptic effect. (2) Legionella cells in cyst encysted amoeba were not killed even at the concentration of 2.5ppm but did at the concentration of 5ppm. (3) Amoeba cells themselves were not killed by the concentration of-5ppm of silver ion.3. The concentration of copper ion in an aqueous solution decreased with a rise in pH.In this study … More , particles coated with copper were added into each aqueous solution with different pH at a final concentration of 0.1%and 0.5%, respectively. Eluted copper concentration was different in 0.1% and 0.5%, however there was no difference among different pH in dissolution rate of copper along with the time course. 4. Antiseptic effect of copper ion against legionella cells in amoeba or amoeba alone was studied.(1) The antiseptic duct of copper ion against legionella cells m trophozoite cells was not shown at the concentration of 1ppm, while legionella cells of 76% at 5ppm and 95% at 10ppm were killed, respectively. (2) The legionella cell in cyst form were not killed at the concentration of 1ppm of copper ion as well as in trophozoite cell. Eighty fie % and 94% of legionella cells were killed at the concentration of 5ppm and 10ppm, respectively. (3) Seventy four % and 47% of amoeba cells survived in the copper ion concentration of 5ppm and 10ppm, respectively.5. In the experiment of antiseptic effect against legionella in copper ion solution with pH7.2, Legionella cells were completely killed at 6h after the addition of 0.5 ppm copper ion. On the other hand, each 0.1% copper solution adjusted. to pH6.0, 7.4 and 8.5 showed all antiseptic effect, and distilled water, tap water and marine water adjusted to copper concentration of 0.1% had also the same effect. The antiseptic effect of hot spring waters against legionella was diversed among each hot spring due to the differences of pH and chemical composition of hot springs. Less

1.研究了温泉的化学成分对银离子杀菌效果的影响。(1)银离子的杀菌效果随着温泉中氯离子和硫酸离子的增加而延迟。(2)一些温泉。尽管银离子浓度足够，但仍没有表现出任何防腐作用，其原因是银离子与某种温泉成分形成络合物。2．对阿米巴体内的军团菌或单独的阿米巴细胞进行了研究。(1)银离子浓度为0.05ppm时，阿米巴体内的军团菌细胞未被杀死，而2.5ppm的银离子则显示出杀菌作用。(2)包囊阿米巴体内的军团菌细胞。即使在2.5ppm的浓度下也不会被杀死，但在5ppm的浓度下会被杀死。 (3)阿米巴细胞本身不会被该浓度杀死。约 5ppm 的银离子。3. 水溶液中铜离子的浓度随着 pH 值的升高而降低。在这项研究中，将涂有铜的颗粒添加到不同 pH 值的水溶液中，最终浓度为 0.1洗脱铜浓度分别为0.1%和0.5%，但不同pH值下铜的溶解率随时间的变化没有差异。研究了铜离子对阿米巴或单独阿米巴体内军团菌的作用。(1)铜离子对军团菌滋养体细胞的抗菌作用在1ppm时不表现出来,而5ppm和95%时铜离子对军团菌细胞的杀菌作用为76%。 (2)在1ppm的铜离子浓度下，包囊形式的军团菌也没有被杀死。在滋养体细胞中，在5ppm和10ppm的浓度下，分别有80%和94%的军团菌细胞被杀死。(3)在5ppm和10ppm的铜离子浓度下，分别有74%和47%的阿米巴细胞存活。 .5.在pH7.2的铜离子溶液中对军团菌的防腐作用实验中，6h时军团菌细胞被完全杀死。添加0.5ppm铜离子后，将各0.1％铜溶液调节至pH6.0、7.4和8.5后均显示出防腐效果，并将蒸馏水、自来水和海水调节至0.1％铜浓度。由于温泉的pH值和化学成分不同，各温泉水对军团菌的杀菌效果也不同。