Effect of exercise training on salivary gland through parasympathetic nervous system

运动训练通过副交感神经系统对唾液腺的影响

基本信息

批准号：
18500516
负责人：
YOSHINO Yoko
金额：
$ 1.03万
依托单位：
Kamakura Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Objective : The objective of the present study was to examine the effect of exercise training on salivary gland through parasympathetic nervous system.Materials and methods: Male ICR mice, weighing 39〜50g, at 32 weeks of age were divided into three groups; an exercise with food restriction (EXP), an exercise without food restriction (EXA), and a control (CTL). EXP group uses voluntary running wheel for exercise, and fed the same amount of diet as the CTL group (pair-feeding). The pilocarpine stimulated whole saliva was collected from the oral cavity by micro-pipet over 15 min 4, 8, and 12 weeks after beginning of the exercise with pair-feeding. The salivary flow rate, protein concentration, and amylase and kallikrein activities were determined. Kallikrein and amylase release are evoked by alpha- and beta- adrenergic receptor stimulation, respectively.Results: The body weight of EXP mice was approximately 20% less than that of CTL mice between 4 and 12 weeks after the beginning of the experiment (p<0.05). Salivary 8-OHdG was significantly increased in the EXP group. The total protein secretion and kallikrein activity decreased 20〜30% in the EXP mice between 4 and 12 weeks after the starting of experiments when compared with the CTL mice(p<0.05〜0.01).Conclusion : The results showed that the combination of exercise and diet control decreased kallikrein activity from the mice saliva, suggesting that signal transduction in the ∂-sympathetic nervous system was down regulated or the synthesis of kallikrein decreased in the salivary glands of exercise mice. The down-regulation was thought to be due to increse of 8-OHdG. This study suggested that the sufficient food intake was necessary for maintaining the oral function during physical exercise.

目的：本研究的目的是通过副交感神经系统检查运动训练对唾液腺的影响。材料和方法：在32周龄时加权39-50g的男性ICR小鼠分为三组；具有食物限制（EXP）的运动，无需食物限制的运动（EXA）和对照（CTL）。 EXP组使用自愿跑步轮进行运动，并喂食与CTL组相同数量的饮食（配对喂养）。毛果石刺激了整个唾液，在锻炼开始时，在15分钟4、8和12周内，通过微水龙头从口腔中收集了整个唾液。确定了唾液流量，蛋白质浓度，淀粉酶和Kallikrein活性。 Kallikrein和淀粉酶释放分别由α-和β-肾上腺素受体刺激引起。回报：EXP小鼠的体重比实验开始后4到12周之间的CTL小鼠小约20％（P <0.05）。 EXP组的唾液8-OHDG显着增加。 The total protein secretion and kallikrein activity decreased 20-30% in the EXP mice between 4 and 12 weeks after the starting of experiments when compared with the CTL mice(p<0.05-0.01).Conclusion: The results shown that the combination of Exercise and diet control decreased kallikrein activity from the mice saliva, suggesting that signal transduction in the ∂-sympathetic nervous system was down regulated or the synthesis of Kallikrein在运动小鼠的唾液腺中得到了改善。下调被认为是由于8-OHDG的增加。这项研究表明，在体育锻炼过程中维持口服功能是必要的。