Effect of exercise training on salivary gland through parasympathetic nervous system

运动训练通过副交感神经系统对唾液腺的影响

基本信息

批准号：
18500516
负责人：
YOSHINO Yoko
金额：
$ 1.03万
依托单位：
Kamakura Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Objective : The objective of the present study was to examine the effect of exercise training on salivary gland through parasympathetic nervous system.Materials and methods: Male ICR mice, weighing 39〜50g, at 32 weeks of age were divided into three groups; an exercise with food restriction (EXP), an exercise without food restriction (EXA), and a control (CTL). EXP group uses voluntary running wheel for exercise, and fed the same amount of diet as the CTL group (pair-feeding). The pilocarpine stimulated whole saliva was collected from the oral cavity by micro-pipet over 15 min 4, 8, and 12 weeks after beginning of the exercise with pair-feeding. The salivary flow rate, protein concentration, and amylase and kallikrein activities were determined. Kallikrein and amylase release are evoked by alpha- and beta- adrenergic receptor stimulation, respectively.Results: The body weight of EXP mice was approximately 20% less than that of CTL mice between 4 and 12 weeks after the beginning of the experiment (p<0.05). Salivary 8-OHdG was significantly increased in the EXP group. The total protein secretion and kallikrein activity decreased 20〜30% in the EXP mice between 4 and 12 weeks after the starting of experiments when compared with the CTL mice(p<0.05〜0.01).Conclusion : The results showed that the combination of exercise and diet control decreased kallikrein activity from the mice saliva, suggesting that signal transduction in the ∂-sympathetic nervous system was down regulated or the synthesis of kallikrein decreased in the salivary glands of exercise mice. The down-regulation was thought to be due to increse of 8-OHdG. This study suggested that the sufficient food intake was necessary for maintaining the oral function during physical exercise.

目的：本研究的目的是探讨运动训练通过副交感神经系统对唾液腺的影响。材料与方法：雄性ICR小鼠，体重39-50g，32周龄，分为三组，一组进行运动训练，一组进行运动训练。限制食物组(EXP)、不限制食物运动组(EXA)和对照组(CTL)使用自愿跑轮进行运动，并喂食与CTL组相同量的饮食。（配对喂养）在开始配对喂养练习后的 15 分钟内，通过微量移液管收集毛果芸香碱刺激的全唾液，并测量唾液流速、蛋白质浓度和唾液浓度。分别测定α-和β-肾上腺素能受体刺激引起的淀粉酶和激肽释放酶活性。结果：体重。实验开始后 4 至 12 周，EXP 小鼠的唾液 8-OHdG 含量比 CTL 小鼠减少约 20%（p<0.05），EXP 组的总蛋白分泌量和激肽释放酶活性降低了 20%。实验开始后4至12周，EXP小鼠与CTL小鼠相比约为30%（p<0.05〜0.01）。结论：结果显示，运动和饮食控制相结合降低了小鼠唾液中激肽释放酶的活性，这表明∂交感神经系统中的信号转导下调或运动调节小鼠唾液腺中激肽释放酶的合成减少。被认为是由于8-OHdG的增加。这项研究表明，在体育锻炼期间，充足的食物摄入对于维持口腔功能是必要的。