Analysis of the distribution and population composition dynamics of red tide-causing microalgae based on their genomic polymorphism

基于基因组多态性的赤潮微藻分布及种群组成动态分析

基本信息

批准号：
17580168
负责人：
YOSHIKAWA Takeshi
金额：
$ 2.04万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The aim of this research is to establish genetic markers to identify a red-tide causing raphidophyte Heterosigma akashiwo intraspecifically.1. The random amplified polymorphic DNA (RAPD) technique detected genomic polymorphism among H. akashiwo strains. Some of sequence-tagged site (STS) primers designed according to the amplicon sequences detected H. akashiwo strains specifically by means of PCR amplification.2. The microsatellite-primed PCR detected genomic polymorphism among H. akashiwo strains. Some of STS primers targeting to the microsatellites found in the amplicon detected H. akashiwo strains specifically by PCR amplification.3. Nucleotide sequences between 16S rDNA and rbcL encoded on the H. akashiwo plastidal DNA, and between rbcL and cfxQ encoded on the plastidal DNA of Chattonella species, which belong to the family Raphidophyceae as well as H. akashiwo, were determined. The Chattonella species showed no sequence differences at the intergenic region between rbcL and rbcS.4. A model of red tide blooms was constructed by adding H akashiwo cells to coastal seawater, and the environmental DNA was prepared. Some of the genetic markers established on the H akashiwo plastidal DNA (Signature A-E, Akase, et. al., 2004) detected H. akashiwo strains specifically from the environmental DNA, suggesting availability of these markers for intraspecific detection of H. akashiwo strains or populations from marine natural environments.

本研究的目的是建立遗传标记，以鉴定引起赤潮的针藻Heterosigma akashiwo的种内特征。 1．随机扩增多态性 DNA (RAPD) 技术检测了 H. akashiwo 菌株的基因组多态性。根据扩增子序列设计的序列标记位点(STS)引物，通过PCR扩增，特异性检测出赤潮嗜血杆菌菌株。 2．微卫星引发的 PCR 检测到了 H. akashiwo 菌株的基因组多态性。扩增子中发现的一些针对微卫星的STS引物通过PCR扩增特异性检测到H. akashiwo菌株。 3．测定了赤潮红藻质体 DNA 上编码的 16S rDNA 和 rbcL 之间的核苷酸序列，以及属于剑藻科和赤潮红藻的查托氏菌属物种质体 DNA 上编码的 rbcL 和 cfxQ 之间的核苷酸序列。 Chattonella 物种在 rbcL 和 rbcS.4 之间的基因间区域没有显示序列差异。通过在沿海海水中添加H akashiwo细胞构建了赤潮水华模型，并制备了环境DNA。在 H akashiwo 质体 DNA 上建立的一些遗传标记（Signature A-E，Akase 等，2004）专门从环境 DNA 中检测到 H. akashiwo 菌株，表明这些标记可用于种内检测 H. akashiwo 菌株或来自海洋自然环境的种群。