Study of metabolic screening, diagnosis, evaluation of treatment, and molecular analysis for organic and fatty acid disorders

有机和脂肪酸疾病的代谢筛查、诊断、治疗评估和分子分析研究

• 批准号: 17390302
• 负责人: YAMAGUCHI Seiji
• 金额: $ 6.23万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390302/
• 关键词: Organic acidemia; fatty acid disorder; mass spectrometry; fast -GC; MS; enzymatic diaanosis; gene analysis; International cooperation; China. Vietnam. India; 有機酸代謝異常; 脂肪酸代謝異常; マススクリーニング; 病因解析; 病態評価; 中国:ベトナム:インド:フランス

Metabolic screening, diagnosis, evaluation of clinical course, and molecular analysis on organic and fatty acid disorders were studied, and the following achievements were obtained.1) Development of the diagnostic support systems for new screening for inherited metabolic disorders of organic and fatty acids: a) A precise method of prenatal diagnosis for organic acid disorders was developed by analysis of amniotic fluid supernatant using two methods, GUMS and Tandem MS, which is very simple and fast; b) The method of enzymatic evaluation of beta-oxidation was developed. The utility of the method was confirmed with analysis of cultured cells from several patients with FAODs.2) Fast-GUMS method: Fast-GC/MS method, using extremely narrow column was developed. The analytical time was cut down to 1/4 of the conventional GUMS using capillary column.3) Cell function of cultured fibroblasts from fatty acid oxidation disorders (FAOD): Cells from FAODs patients such as VLCAD deficiency (VLCADD), MCAD deficiency (MCADD), CPU deficiency and glutaric acidemia type 2 (GA2) were cultured in the condition of high temperature, and it was revealed that oxidation of medium chain fatty acids was accelerated, while that of longer chain fatty acids was inhibited. It suggested that FAOD patients potentially have a risk of acute encephalopathy in the febrile condition. On the other hand, metabolic condition of FAOD cells was improved by Bezafibrate, a hypolipidemic drug.4) Molecular basis of Japanese FAODs: Over 10 Japanese patients with VLCADD and GA2 were analyzed at the molecular levels. Genotypes of these diseases were heterogeneous in Japanese.5) Study of etiology of diseases and effect of newborn screening using tandem MS: It was suggested that he proportion of diseases between Japanese and the other Asian counties was different. Outcomes between patients detected by newborn screening was much better than those detected after onset stage.

对有机脂肪酸代谢性疾病的代谢筛查、诊断、临床病程评估、分子分析等方面进行了研究，取得了以下成果。1）遗传性有机脂肪酸代谢性疾病新型筛查诊断支持系统的开发： a) 通过使用GUMS和Tandem MS两种方法分析羊水上清液，开发出一种精确的有机酸紊乱产前诊断方法，非常简单、快速； b) 开发了β-氧化的酶促评价方法。通过对几位FAODs患者的培养细胞的分析，证实了该方法的实用性。2）Fast-GUMS方法：开发了使用极窄色谱柱的Fast-GC/MS方法。使用毛细管柱，分析时间缩短至传统GUMS的1/4。3）脂肪酸氧化障碍（FAOD）培养的成纤维细胞的细胞功能：来自FAODs患者的细胞，例如VLCAD缺乏症（VLCADD），MCAD缺乏症（MCADD） ），CPU缺乏症和2型戊二酸血症（GA2）在高温条件下培养，发现中链脂肪酸的氧化加速，而长链脂肪酸的氧化加速。酸被抑制。这表明FAOD患者在发热状态下可能有发生急性脑病的风险。另一方面，降血脂药物苯扎贝特改善了FAOD细胞的代谢状况。4）日本FAOD的分子基础：对10多名日本VLCADD和GA2患者进行了分子水平分析。这些疾病的基因型在日本人中存在异质性。5）使用串联MS进行疾病病因学和新生儿筛查效果的研究：表明日本与其他亚洲国家之间的疾病比例不同。通过新生儿筛查发现的患者之间的结果比发病后发现的结果要好得多。

项目成果

Significance of newborn mass screening for fatty acid disorders to prevent children from sudden infant death or acute encephalopathyof unknown origin

新生儿大规模筛查脂肪酸紊乱对预防儿童婴儿猝死或不明原因急性脑病的意义

• 发表时间: 2005
• 作者: Yamaguchi;S;Yamaguchi S;Yamaguchi S;Yamaguchi S;Yamaguchi S
• 通讯作者: Yamaguchi S

Survey of Mitochondrial Fatty Acid Oxidation Disorders (FAODs) (in Japanese)

线粒体脂肪酸氧化障碍 (FAOD) 调查（日语）

• 发表时间: 2005
• 作者: Yamaguchi;S;Yamaguchi S;Yamaguchi S
• 通讯作者: Yamaguchi S

今日の小児治療指針 第14版 : ミトコンドリアβ酸化異常症

今日儿科治疗指南第 14 版：线粒体 β-氧化障碍

• 发表时间: 2006
• 作者: Yamaguchi;S;山口清次;山口清次;山口清次
• 通讯作者: 山口清次

Inherited metabolic disorder of organic and fatty acids : A causative disorder of unexpected acute encephalopathy or sudden death in childhood

遗传性有机酸和脂肪酸代谢紊乱：导致儿童意外急性脑病或猝死的疾病

• 发表时间: 2006
• 作者: Yamaguchi;S
• 通讯作者: S

Prenatal diagnosis for organic acid disorders using two mass spectrometric methods, gas chromatography mass spectrometry and tandem mass spectrometry

• DOI: 10.1016/j.jchromb.2005.04.020
• 发表时间: 2005-08-25
• 期刊: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
• 影响因子: 3
• 作者: Hasegawa, Y;Iga, M;Yamaguchi, S
• 通讯作者: Yamaguchi, S