Isolation of genes determining the flower color traits in Asiatic hybrid lily and their characterization by RNAi

亚洲杂交百合花色性状基因的分离及其 RNAi 表征

基本信息

批准号：
17380015
负责人：
YAMAGISHI Masumi
金额：
$ 10.84万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380015/
关键词：
Asiatic hybrid lily Anthocyanins Carotenoids Flower color Gene expression QTL mapping Cultivar identity RNAi スカシュリ遺伝子現

项目摘要

Because anthocyanin pigmentation in tepals of Asiatic hybrid lily is controlled by a single dominant gene and anthocyanin biosynthetic genes are expressed coordinately in tepals, some transcription factor is expected to control the presence or absence of anthocyanin pigment. In this study, two genes, LhbHLH1 and LhbHLH2 were isolated, which had homologies with PhJAF13 and PhAN1, respectively. LhbHLH1 and LhbHLH2 were expressed in tepals and leaves. When lily buds were exposed to light, anthocyanin pigment accumulated in leaves, which nearly disappeared 8 days after light exposure. Expression of LhbHLH2 was strongly affected by light, which peaked at 2 days after light exposure and decreased after that. This expressional change was nearly parallel with the change of anthocyanin content. Similar result was observed in tepals, that is, both anthocyanin content and LhbHLH2 expression were decreased by the dark treatment. These results suggest that the two bHLH genes, especially LhbHLH2, ar … More e correlating to anthocyanin biosynthesis. Next, carotenoid composition and the expression of genes encoding carotenoid biosynthetic enzymes were analyzed. Most of the carotenoids in yellow petals were antheraxanthin, 9Z-violaxanthin, cis-lutein and violaxanthin, and the major carotenoid in red petal was capsantin. Tepals of yellow and red flowered cultivars showed increased accumulation of carotenoids, whereas carotenoid content was decreased in those of white and pink flowered cultivars. Carotenoid biosynthesizing genes, PSY, PDS, ZDS, CRTISO, LCYB, HYB and LCYE, were isolated and their expression was examined. Transcription of PSY, PDS, ZDS, CRTISO, and HYB increased as tepals grew and peaked at the 6-7 cm tepal-length stage. On the contrary, transcription of LCYB and LCYE were constant and decreased, respectively, during tepal development. Transcription patterns of these genes were basically the same among yellow, red, pink and white colored tepals although carotenoid contents were very different among them, indicating that carotenoid accumulation in tepals is not explained by the transcription of biosynthetic genes. Less

由于亚洲杂种百合花的花色素色素沉着由单个显性基因控制，而花色素的生物合成基因在阵发中协调表达，因此某些转录因子有望控制或不存在青色蛋白色素的存在或不存在。在这项研究中，分离了两个基因LHBHLH1和LHBHLH2，分别具有PHJAF13和PHAN1的同源性。 LHBHLH1和LHBHLH2在全磁和叶子中表达。当百合芽暴露于光线时，叶子中积聚了花色苷颜料，暴露于光线后8天几乎消失。 LHBHLH2的表达受光的强烈影响，光在光照后2天达到峰值，此后增加。这种表达变化几乎与花青素含量的变化相似。在Tepals中观察到了相似的结果，即，通过黑暗处理，绿o蛋白含量和LHBHLH2表达都得到了改善。这些结果表明，两个BHLH基因，尤其是LHBHLH2，AR…与花青素生物合成更相关。接下来，分析了类胡萝卜素组成和编码类胡萝卜素生物合成酶的基因的表达。黄色花瓣中的大多数类胡萝卜素是动脉粥样硬糖，9z-violaxanthin，cis-lutein和fiparaxanthin，红色花瓣中的主要类胡萝卜素是辣椒。黄色和红色花品种的毛发显示类胡萝卜素的积累增加，而在白色和粉红色的花品种中，类胡萝卜素含量减少了。分离出类胡萝卜素生物合成基因，PSY，PDS，ZDS，CRTISO，LCYB，HYB和LCYE，并检查了它们的表达。随着Tepals在LCYB和LCYE的6-7 cm转录下的增长和峰值，PSY，PDS，ZDS，CRTISO和HYB的转录分别恒定和改进，在阵发发育过程中。这些基因的转录模式基本上是在黄色，红色，粉红色和白色的Tepals中相同的，尽管类胡萝卜素含量在它们中有很大不同，这表明生物合成基因的转录并不能解释小链球类胡萝卜素的积累。较少的