Isolation of genes determining the flower color traits in Asiatic hybrid lily and their characterization by RNAi

亚洲杂交百合花色性状基因的分离及其 RNAi 表征

基本信息

批准号：
17380015
负责人：
YAMAGISHI Masumi
金额：
$ 10.84万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380015/
关键词：
Asiatic hybrid lily Anthocyanins Carotenoids Flower color Gene expression QTL mapping Cultivar identity RNAi スカシュリ遺伝子現

项目摘要

Because anthocyanin pigmentation in tepals of Asiatic hybrid lily is controlled by a single dominant gene and anthocyanin biosynthetic genes are expressed coordinately in tepals, some transcription factor is expected to control the presence or absence of anthocyanin pigment. In this study, two genes, LhbHLH1 and LhbHLH2 were isolated, which had homologies with PhJAF13 and PhAN1, respectively. LhbHLH1 and LhbHLH2 were expressed in tepals and leaves. When lily buds were exposed to light, anthocyanin pigment accumulated in leaves, which nearly disappeared 8 days after light exposure. Expression of LhbHLH2 was strongly affected by light, which peaked at 2 days after light exposure and decreased after that. This expressional change was nearly parallel with the change of anthocyanin content. Similar result was observed in tepals, that is, both anthocyanin content and LhbHLH2 expression were decreased by the dark treatment. These results suggest that the two bHLH genes, especially LhbHLH2, ar … More e correlating to anthocyanin biosynthesis. Next, carotenoid composition and the expression of genes encoding carotenoid biosynthetic enzymes were analyzed. Most of the carotenoids in yellow petals were antheraxanthin, 9Z-violaxanthin, cis-lutein and violaxanthin, and the major carotenoid in red petal was capsantin. Tepals of yellow and red flowered cultivars showed increased accumulation of carotenoids, whereas carotenoid content was decreased in those of white and pink flowered cultivars. Carotenoid biosynthesizing genes, PSY, PDS, ZDS, CRTISO, LCYB, HYB and LCYE, were isolated and their expression was examined. Transcription of PSY, PDS, ZDS, CRTISO, and HYB increased as tepals grew and peaked at the 6-7 cm tepal-length stage. On the contrary, transcription of LCYB and LCYE were constant and decreased, respectively, during tepal development. Transcription patterns of these genes were basically the same among yellow, red, pink and white colored tepals although carotenoid contents were very different among them, indicating that carotenoid accumulation in tepals is not explained by the transcription of biosynthetic genes. Less

由于亚洲杂交百合花青素色素的沉着是由单个显性基因控制的，并且花青素生物合成基因在花青素生物合成基因在花被片中协调表达，因此预计某些转录因子可以控制花青素色素的存在或不存在。在本研究中，两个基因LhbHLH1和分离出LhbHLH2，其分别与PhJAF13和PhAN1具有同源性。 LhbHLH2在花被片和叶片中表达，当百合芽受光时，叶片中花青素积累，受光影响后8天，LhbHLH2的表达量达到高峰，受光后2天表达量下降。这种表达变化几乎与花青素含量的变化在花被片中观察到类似的结果，即花青素含量和花青素含量的变化。这些结果表明，与花青素生物合成相关的两个 bHLH 基因，尤其是 LhbHLH2 基因的表达减少。接下来，对类胡萝卜素的组成和编码类胡萝卜素生物合成酶的基因的表达进行了分析。黄色花瓣中含有蒽黄素、9Z-紫黄素、顺式叶黄素和黄花和红花品种花被中类胡萝卜素含量增加，而红花瓣中主要类胡萝卜素为辣椒红，而白花和粉花品种花被类胡萝卜素生物合成基因PSY、PDS、ZDS、CRTISO的类胡萝卜素含量减少。、LCYB、HYB 和 LCYE，被分离并检查它们的 PSY、PDS、的转录。 ZDS、CRTISO和HYB随着花被片的生长而增加，并在花被片长度6-7 cm阶段达到顶峰，相反，LCYB和LCYE的转录在花被片发育过程中分别保持恒定和减少。尽管黄色、红色、粉色和白色花被片中的类胡萝卜素含量差异很大，但黄色、红色、粉红色之间的情况相同，这表明花被片中类胡萝卜素的积累不能用以下转录来解释：生物合成基因较少。