Creation Technology of Nano-mechanisms using Particle Positioning on DNA Strands

利用 DNA 链上的粒子定位的纳米机制创建技术

基本信息

批准号：
17360112
负责人：
HATSUZAWA Takeshi
金额：
$ 10.16万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17360112/
关键词：
DNA Nano-Machine Nano-Bio Molecular Mechanism

项目摘要

In this research, a new technology for creation of nano-mechanisms using DNA and related biotechnology has been investigated and obtained the following results.1.Periodic construction of nano-mechanisms by DNA: Nano-particle modified DNA double strand fractions have been created and ligated (connected each other by enzyme) for several tens of blocks. Since the pitch of the DNA nucleic acid is 0.34nm, the positioning resolution is very fine compare to those of mechanically obtained. Experimental results show the periodic nano-structure by the optimization of the sequence of the nucleic acid, which are observed by the AFM.2.Creation of Molecular nano-mechanisms by protein complex: To add the function such as actuators and sensors, a complex with DNA and protein gas been proposed. A DNA folding protein called IHF has been introduced to bend the DNA at designated nucleic sequence, and experimentally confirmed by AM micrographs. Also, a protein ReccA has been introduced to branch DNA at the hybridized sequence, which is experimentally confirmed as nano-junctions.3.Nano-structures by circular DNA and Mechanical Property Evaluation of DNA : Three types of nano-structure, DNA glasses, Serial DNA rings, and DNA chain has been proposed and experimentally fabricated. A DNA glasses with a diameter of 27nm has been formed and confirmed by AFM. A mechanical model of the DNA has been proposed by combining coil spring for main structure and small springs for nucleic and hydrogen bonding, which is compared with the experimental results and show good agreement.

本研究对利用DNA和相关生物技术构建纳米机制的新技术进行了研究，并取得了以下成果： 1.利用DNA周期性构建纳米机制：创建了纳米颗粒修饰的DNA双链片段，并进行了研究。连接（通过酶相互连接）数十个区块。由于DNA核酸的节距为0.34nm，因此与机械获得的定位分辨率相比非常精细。实验结果通过优化核酸序列呈现周期性纳米结构，并通过AFM观察到。2.通过蛋白质复合物创建分子纳米机制：添加执行器和传感器等功能，复合物提出了用DNA和蛋白质气体。一种称为 IHF 的 DNA 折叠蛋白已被引入，以在指定的核酸序列处弯曲 DNA，并通过 AM 显微照片进行实验证实。此外，在杂交序列的分支DNA中引入了蛋白质ReccA，经实验证实其为纳米结。3.环状DNA的纳米结构和DNA的机械性能评估：三种类型的纳米结构、DNA玻璃、连续DNA环和DNA链已被提出并通过实验制造。直径为27nm的DNA玻璃已形成并经AFM证实。结合主要结构的螺旋弹簧和用于核酸和氢键的小弹簧，提出了DNA的力学模型，与实验结果进行了比较，显示出良好的一致性。