Phylogenetic analysis of avian retroviruses with neuropathogenicity in central nervous system

中枢神经系统致病性禽逆转录病毒的系统发育分析

• 批准号: 16405033
• 负责人: OCHIAI Kenji
• 金额: $ 7.42万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16405033/
• 关键词: fowl; glioma; central nervous system; phylogenetic analysis; retrovirus; avian leukosis virus (ALV); グリオーマ

Avian leukosis virus (ALV) mainly causes hematopoietic neoplasms in chicken, whereas fowl glioma-inducing virus (FGV), which belongs to ALV subgroup A, induces astrocytoma in chickens. Fowls kept in zoological garden A in Toyama have been considered as source for the FGV infection in other Japanese zoological gardens. However, the background and process how FGV acquired the unique oncogenicity in nervous system has not been clarified. At first, to detect FGV using nested PCR, primers were designed specific to the 3' untranslated region (UTR) and the PCR-protocol was established. The prevalence of FGV by the nested PCR in 131 Japanese fowls of zoological garden A was 40% and the prevalences in a total of 129 chickens in three other zoological gardens, which were received the eggs or chicks from the zoological garden A, were 26 to 56%. Phylogenetic analysis revealed that 3' UTRs, including the FGV-specific sequence, of the 14 isolated ALVs showed high sequence identity and a close relati … More onship with that of FGV. Secondly, the prevalence in chickens, which were not related by birth to any fowls of the zoological garden A, was examined. Ancestors of Japanese fowl are thought to be brought to Japan in the Edo era from Southeast Asia. Along the propagation path, feather pulps and cloaca swab were collected from 264 chickens in Indonesia, Philippines, South Korea and Japan. Twenty nine chickens, including 9 birds in the foreign countries, were positive for nested PCR and the amplified products of showed 98% nucleotide sequence homology to the corresponding region of FGV. Six strains of FGV were isolated from these chickens in Japan. On the other hand, different ALV strain (Tyms_90) was isolated from brains of layers affected with fowl glioma and subcutaneous mesenchymal neoplasm in Japan. Another two Japanese fowls in Japan showed perineurioma and neurofibroma, respectively and a strain of ALV having endogenous-1(ev-1) locus was isolated from the latter. These results suggest that mutants of FGV spread in Japanese fowls in Japan, that small number of domestic and foreign chickens unrelated to the birds in the zoological garden A is infected with FGV, and that Japanese fowls are infected with other ALVs having ev-1 locus in the genome and showing oncogenicity in nervous system. In conclusion, it is speculated that FGV has appeared outside of the zoological garden A and a part of FGV-infected chickens were brought to this garden and used for breeding. Less

禽白血病病毒（ALV）主要引起鸡的造血系统肿瘤，而禽神经胶质瘤诱导病毒（FGV）属于ALV A亚组，可诱发鸡的星形细胞瘤。富山A动物园饲养的鸡被认为是该病的来源。日本其他动物园的FGV感染情况 然而，FGV如何获得神经系统独特的致癌性的背景和过程尚不清楚。首先，为了使用巢式PCR检测FGV，设计了针对3'非翻译区(UTR)的特异引物并建立了PCR方案。通过巢式PCR在A动物园的131只日本家禽中FGV的患病率为40%。其他三个动物园的129只鸡接受了A园鸡蛋或雏鸡的患病率为26％至56％。系统发育分析显示，14 个分离的 ALV 的 3' UTR（包括 FGV 特异性序列）显示出较高的序列同一性，并且与 FGV 的序列密切相关。其次，鸡中的流行情况与出生无关。动物园里的任何家禽都被认为是江户时代从东南亚带到日本的。采集印度尼西亚、菲律宾、韩国、日本264只鸡泄殖腔拭子，巢式PCR阳性29只，其中国外9只，扩增产物与相应区域核苷酸序列同源性达98%。 FGV 从日本的这些鸡中分离出 6 株 FGV。另一方面，从患有家禽神经胶质瘤的蛋鸡脑中分离出不同的 ALV 株（Tyms_90）。日本的另外两只日本禽分别表现出神经周瘤和神经纤维瘤，并且从后者中分离出具有内源性1（ev-1）基因座的ALV株。这些结果表明FGV的突变体在日本传播。日本家禽中，少数与 A 动物园鸟类无关的国内外鸡感染了 FGV，日本家禽也感染了其他 ALV 病毒ev-1位点存在于基因组中，并在神经系统中显示出致癌性。综上所述，推测FGV已出现在A动物园之外，并且部分感染FGV的鸡被带到该花园并用于繁殖。

项目成果

Persistent prevalence in Japan of fowl glioma-inducing virus with a genome mutation in the env gene.

在日本，具有 env 基因突变的禽神经胶质瘤诱导病毒持续流行。

• 发表时间: 2008
• 期刊: Journal of Veterinary Medical Science 70(In press)
• 作者: Hatai;H.;Ochiai;K.;et. al.
• 通讯作者: et. al.

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

• 发表时间: 2005
• 作者: Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
• 通讯作者: 星野 幹雄

Pathology of naturally occurring multiple perineuriomas in a chicken

鸡自然发生的多发性神经束膜瘤的病理学

• 发表时间: 2007
• 作者: Ochi;A.;Ochiai;K. ;et. al.
• 通讯作者: et. al.

Nested polymerase chain reaction for detection of the avian leucosis virus causing so-called fowl glioma

巢式聚合酶链反应用于检测引起所谓家禽神经胶质瘤的禽白血病病毒

• 发表时间: 2005
• 期刊: Avian Pathology 34(6)
• 作者: Hatai;H.;Ochiai;K.et al.
• 通讯作者: K.et al.

Epidemiological study on so-called fowl glioma-prevalence in Japan and the mutation of the causal virus-

所谓禽神经胶质瘤的流行病学研究-日本的流行情况及其致病病毒的变异-

• 发表时间: 2005
• 作者: Hatai;H.;Ochiai;K. ;et. al.
• 通讯作者: et. al.