Immunoscanning electron microscopic study on the localization of lymphangiogenic factor VEGF-C in oral

口腔淋巴管生成因子VEGF-C定位的免疫扫描电镜研究

• 批准号: 16390596
• 负责人: SUGIHARA Kazumasa
• 金额: $ 4.8万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390596/
• 关键词: oral sqt amous cell carcinoma; lymphangiogenesis; VEGF-C; lymph node metastasis; immunohistochemistry; CD-105; 所属リンパ節転移; CD105; 免疫走査電顕法

Lymphatic vessel density (LVD) and microvessel density (MVD) are important parameters for assessing tine malignant potential of oral squamous cell carcinoma (OSCC) and patient survival. This study aims to clarify the clinical and prognostical significances of LVD and MVD in OSCC, and the lymphagiogenic and angiogenic activities of VEGF-C in cancer tissues. A total of 110 OSCC tissues were evaluated for LVD, MVD and expression of VEGF-C using immunohistochemistry. Correlations among these parameters and clinicpopathologic factors were examined. LVD was significantly higher in tumors with a very high expression of VEGF-C than in those with no/week expression of VEGF-C. LVD correlated well with lymph node metastasis. MVD significantly correlated with positive lymph node metastasis, but did not with VEGF-C expression. In contrast, high expression of VEGF-C significantly correlated with advanced tumor status. Survival rate was lower in patients with a higher LVD, higher MVD and strong VEGF-C expression. Lymphangiogenesis most predominantly influences metastasis-free survival. Our results suggested that LVD is a more useful tool than MVD and VEGF-C for deciding on therapeutic strategies for OSCC.

淋巴血管密度（LVD）和微血管密度（MVD）是评估口服鳞状细胞癌（OSCC）和患者存活的重要参数。这项研究旨在阐明OSCC中LVD和MVD的临床和预后意义，以及VEGF-C在癌症组织中VEGF-C的淋巴胆料生成和血管生成活性。使用免疫组织化学评估了总共110个OSCC组织的LVD，MVD和VEGF-C的表达。研究了这些参数和临床病理因素之间的相关性。 VEGF-C表达非常高的肿瘤中LVD明显高于无/周表达VEGF-C的LVD。 LVD与淋巴结转移良好相关。 MVD与阳性淋巴结转移显着相关，但与VEGF-C表达无关。相反，VEGF-C的高表达与晚期肿瘤状态显着相关。 LVD，MVD较高和强烈VEGF-C表达的患者的存活率较低。淋巴管生成最多主要影响无转移的生存。我们的结果表明，LVD比MVD和VEGF-C更有用，用于决定OSCC的治疗策略。

项目成果

Tumor lymphangiogenesis correlates with lymph node metastasis and clinicopathologic parameters in oral squamous cell carcinoma

• DOI: 10.1002/cncr.22900
• 发表时间: 2007-09-15
• 期刊: CANCER
• 影响因子: 6.2
• 作者: Miyahara, Mayumi;Tanuma, Jun-ichi;Semba, Ichiro
• 通讯作者: Semba, Ichiro

Freeze-fracture observations on the membranes and junctions in human and oral squamous cell carcinoma

人类和口腔鳞状细胞癌膜和连接处的冷冻断裂观察

• 发表时间: 2005
• 期刊: 日本口腔診断学会雑誌 18・2
• 作者: Seishi Kawano;Kazumasa Sugihara
• 通讯作者: Kazumasa Sugihara

Freeze-fracture observation on the membranes and junctions in human normal and oral squamous cell carcinoma

人正常和口腔鳞状细胞癌膜和连接处的冷冻断裂观察

• 发表时间: 2005
• 期刊: Japanese journal of oral diagnosis/oral medicine Vol.18, NO.2
• 作者: Seishi kawano;kazumasa Sugihara
• 通讯作者: kazumasa Sugihara