Mechanism of biological clock in mammals: Analysis of regulatory mechanism of

哺乳动物生物钟机制：调控机制分析

• 批准号: 16380201
• 负责人: MURAKAMI Noboru
• 金额: $ 10.05万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380201/
• 关键词: Biological clock; Neuromedin U; Neuromedin S; Suprachiasmatic nucleus; Circadian rhythm; 位相変位; 時計関連遺伝子; 新規ペプチド; 概日リズム

We identified a novel neuropeptide of 36 amino-acid residues in rat brain as an endogenous ligand for the orphan G protein-coupled receptor FM-4/TGR-1, which was identified to date as the neuromedin U (NMU) receptor, and designate this peptide 'neuromedin S (NMS)' because it is specifically expressed in the suprachiasmatic nuclei (SCN) of the hypothalamus. We examined the role of NMU and NMS on the biological rhythm. The expression of NMU mRNA exhibited a circadian rhythm, with the peak expression in the SCN occurring at CT4-8 h. The two NMU-binding receptors (NMU-R1 and NMU-R2) were also expressed in the SCN Intracerebroventricular injection (ICV) of NMU and NMS induced the expression of Fos protein in the SCN cells and caused a phase-dependent phase shift of the circadian loco motor activity rhythm. This NMU-and NMS-induced phase shift was of the nonphotic type. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed increases in the expression in the SCN of immediate early genes, such as c-fos, NGFI-A, NGFI-B, and JunB. Furthermore, ICV injection of NMU increased the expression of Per1, but not Per2 in the SCN. These results indicate that NMU and NMS may play some important role in the circadian oscillator by exerting an autocrine or paracrine action in the SCN.

我们确定了大鼠脑中36个氨基酸残基的新型神经肽是孤儿G蛋白偶联受体FM-4/TGR-1的内源配体，迄今已鉴定为神经蛋白U（NMU）受体，并指定为指定。该肽“神经蛋白S（NMS）”是因为它是在下丘脑的上张核（SCN）中表达的。我们研究了NMU和NMS在生物节奏中的作用。 NMU mRNA的表达表现出昼夜节律，SCN的峰表达发生在CT4-8 h。在NMU和NMS的SCN室内注射（ICV）中，两个NMU结合受体（NMU-R1和NMU-R2）也表达昼夜节律运动节奏节奏。这种NMU和NMS诱导的相移为非晶状体类型。定量逆转录酶 - 聚合酶链反应（RT-PCR）分析显示，直接早期基因的SCN表达增加，例如C-FOS，NGFI-A，NGFI-B和JUNB。此外，ICV注射NMU会增加PER1的表达，但在SCN中不增加PER2。这些结果表明，NMU和NM可以通过在SCN中发挥自分泌作用或旁分泌作用来在昼夜节律振荡器中起重要作用。

项目成果

Neuromedin S is a novel anorexigenic hormone

• DOI: 10.1210/en.2005-0107
• 发表时间: 2005-10-01
• 期刊: ENDOCRINOLOGY
• 影响因子: 4.8
• 作者: Ida, T;Mori, K;Murakami, N
• 通讯作者: Murakami, N

Identification of neuromedin S and its possible role in the mammalian circadian oscillator system

• DOI: 10.1038/sj.emboj.7600526
• 发表时间: 2005-01-26
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Mori, K;Miyazato, M;Kangawa, K
• 通讯作者: Kangawa, K

Maternal ghrelin plays an important role in fetal development during

母体ghrelin在胎儿发育过程中发挥着重要作用

• 发表时间: 2006
• 期刊: Endocrinology 147(3)
• 作者: Ueno M.;Katayama K.;Yamauchi H.;Nakayama H.;Doi K.;Katayama K. et al.;T.Ida;M.Sato;S.Shousha;P.Verbeek;P.Verbeek;T.Ida;S.Shousha;S Shousha;K Nakahara;M Sato;H.Sawada;Y Date;K Nakahara;Toshinai K;H.Sawada;Y Date;K Nakahara;M Sato;Y Date;K Nakahara
• 通讯作者: K Nakahara

Effect of neuromedin S on feeding regulation in the Japanese quail

• DOI: 10.1016/j.neulet.2005.08.033
• 发表时间: 2006-01
• 期刊: Neuroscience Letters
• 影响因子: 2.5
• 作者: S. Shousha;K. Nakahara;Miho Sato;K. Mori;M. Miyazato;K. Kangawa;N. Murakami

臨床分子内分泌学

临床分子内分泌学

• 发表时间: 2004
• 作者: 村上 昇;中原桂子;寒川賢治
• 通讯作者: 寒川賢治